NESbase version 1.0, a database of Nuclear Export Signals.
Tanja la Cour2,1, Ramneek Gupta1,
Karen Skriver2, Flemming M. Poulsen2
and Søren Brunak1.
Nucleic Acids Res., 31(1):393-6, 2003.
Center for Biological Sequence Analysis, Technical University of Denmark,
DK-2800 Lyngby, Denmark
Department of Protein Chemistry, Institute of Molecular Biology,
University of Copenhagen, DK-1353 Copenhagen, Denmark
Protein export from the nucleus is often mediated by a Leucine-rich
nuclear export signal. NESbase is a database of experimentally validated
Leucine-rich Nuclear Export Signals (NES) curated from literature. These
signals are not annotated in databases such as SWISS-PROT, PIR or PROSITE.
Each NESbase entry contains information of whether NES was shown to be
necessary and/or sufficient for export, and whether the export was shown
to be mediated by the export receptor CRM1. The compiled information was
used to make a sequence logo of the Leucine-rich NESs, displaying the
conservation of amino acids within a window of 25 residues. Surprisingly,
only 36% of the sequences used for the logo fit the widely accepted NES