NESbase 1.0 format description

The database is in HTML; it is downloadable as text without any loss of information other than the hyperlinks to Swiss-Prot and Medline. It is organized in entries, one per protein. Each entry has the following fields:
NES-accession	NESbase accession code e.g. NES-0001
Date	Dates for creation and updates of entry.
Protein	Protein description.
Organism	Species name e.g. Homo sapiens
DB_reference	Sequence Cross-reference to Swiss-Prot, PIR or Genbank.
Necessity	Experimental evidence (if any), indicating that the signal is necessary for export. Usually shown by deletion of or mutations in the signal that greatly impairs or abrogates export.
Sufficiency	Experimental evidence (if any), indicating that the signal is nsufficient for export. Usually shown by a peptide bearing the NES-signalp mediating export of a reporter protein.
Pathway	LMB-sensitivity and/or CRM1-dependency if described.
Location	Nucleocytoplasmic shuttling proteins are often predominantly localised to either nucleus or cytoplasm at steady state. When available, information of steady state localisation is given in this field.
Regulation	Export can be regulated, for instance, by a change in phosphorylation state of the protein or by protein-protein interaction masking the NES signal. When available, information of regulation is given in this field.
Comments	Other relevant information.
References	Literature references and Medline links.
Sequence	Sequence followed by an assignment field. The assignment field reflects the experimental data described in the necessity and sufficiency fields: Point-mutations that greatly impair export either alone or together are designated "M"; Sequence segments shown to mediate export of either truncations of the protein being tested or of a reporter protein are designated "a".

GETTING HELP