NESbase 1.0 format description
The database is in HTML; it is downloadable as text without any
loss of information other than the hyperlinks to Swiss-Prot and
Medline. It is organized in entries, one per protein. Each
entry has the following fields:
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NES-accession
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NESbase accession code e.g. NES-0001
Date
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Dates for creation and updates of entry.
Protein
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Protein description.
Organism
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Species name e.g. Homo sapiens
DB_reference
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Sequence Cross-reference to Swiss-Prot, PIR or Genbank.
Necessity
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Experimental evidence (if any), indicating that the signal is
necessary for export. Usually shown by deletion of or
mutations in the signal that greatly impairs or abrogates export.
Sufficiency
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Experimental evidence (if any), indicating that the signal is
nsufficient for export. Usually shown by a peptide bearing
the NES-signalp mediating export of a reporter protein.
Pathway
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LMB-sensitivity and/or CRM1-dependency if described.
Location
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Nucleocytoplasmic shuttling proteins are often predominantly localised
to either nucleus or cytoplasm at steady state. When available,
information of steady state localisation is given in this field.
Regulation
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Export can be regulated, for instance, by a change in phosphorylation
state of the protein or by protein-protein interaction masking the
NES signal. When available, information of regulation is given in this
field.
Comments
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Other relevant information.
References
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Literature references and Medline links.
Sequence
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Sequence followed by an assignment field. The assignment field reflects
the experimental data described in the necessity and sufficiency fields:
- Point-mutations that greatly impair export either alone or
together are designated "M";
- Sequence segments shown to mediate export of either truncations
of the protein being tested or of a reporter protein are designated
"a".
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