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NESbase 1.0 format description

The database is in HTML; it is downloadable as text without any loss of information other than the hyperlinks to Swiss-Prot and Medline. It is organized in entries, one per protein. Each entry has the following fields:

NES-accession NESbase accession code e.g. NES-0001
Date Dates for creation and updates of entry.
Protein Protein description.
Organism Species name e.g. Homo sapiens
DB_reference Sequence Cross-reference to Swiss-Prot, PIR or Genbank.
Necessity
Experimental evidence (if any), indicating that the signal is necessary for export. Usually shown by deletion of or mutations in the signal that greatly impairs or abrogates export.
Sufficiency
Experimental evidence (if any), indicating that the signal is nsufficient for export. Usually shown by a peptide bearing the NES-signalp mediating export of a reporter protein.
Pathway LMB-sensitivity and/or CRM1-dependency if described.
Location
Nucleocytoplasmic shuttling proteins are often predominantly localised to either nucleus or cytoplasm at steady state. When available, information of steady state localisation is given in this field.
Regulation
Export can be regulated, for instance, by a change in phosphorylation state of the protein or by protein-protein interaction masking the NES signal. When available, information of regulation is given in this field.
Comments Other relevant information.
References Literature references and Medline links.
Sequence Sequence followed by an assignment field. The assignment field reflects the experimental data described in the necessity and sufficiency fields:
  • Point-mutations that greatly impair export either alone or together are designated "M";
  • Sequence segments shown to mediate export of either truncations of the protein being tested or of a reporter protein are designated "a".



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