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27621 - Prokaryotic Gene Discovery, Metagenomics and Pangenomics

Course Programme - Autumn 2009

Practical Information


The aim of this course is to give the students a solid knowledge of methods for determining microbial meta-, core- and pan-genomes, based on the genome sequences of a set of related organisms or microbial communities. They should be able to understand and apply this information to practical uses, such as screening large segments of sequenced DNA extracted from wide-ranging environmental samples, classification of bacterial species as well as the design of high-density microarray chips. Furthermore, it is a goal that the students learn to formulate interesting problems/questions that can be addressed by pangenome-chip and advanced metagenomics analysis.

Lectures and exercises will take place at the Technical University of Denmark, building 208, room 062, in Lyngby.

Exam

Written Exam, 4 hours
Date: 10-12-2009

Excercises

Mail the lab/exercise reports to exercises27621@cbs.dtu.dk
Check out the Exercise Checklist to see which exercises you are missing.

Reading material:

Exact details as to which chapters and which papers are considered curriculum will be given as the course progresses.

Software and services used in some exercises:


Lecture Schedule


O Monday, August 31
13:00-?
Course Introduction (Slides)
Carsten Friis
Exercise 1: Read DNA sequencing: bench to bedside and beyond and write a very short resume (timeline and highlights) - deadline: Thursday, September 3.
Mail the text to exercises27621@cbs.dtu.dk
 
O Thursday, September 3
13:00-13:45
History and basics of DNA sequencing (Slides)
Thomas Sicheritz-Ponten
14:00-17:00
Exercise 2: Sanger Sequencing Runs (link to the Sanger Method Animation)
Thomas Sicheritz-Ponten
 
O Monday, September 7
13:00-13:45
Introduction to Pan- and Meta-genomics (Slides)
Dave Ussery
14:00-14:45
DNA Structure
Dave Ussery
15:00-17:00
Exercise 3: DNA Atlases (Slides)
Dave Ussery
 
O Thursday, September 10
13:00-13:45
A short introduction to Molecular Phylogeny
Anders Gorm Petersen
13:45-17:00
Student presentations of sequencing techniques
Thomas Sicheritz-Ponten
 
O Monday, September 14
13:00-15:00
Who's in there - 16S analysis (slides for lecture and lab)
Thomas Sicheritz-Ponten
15:00-17:00
Exercise 4: Diversity analysis - RNAmmer, Greengenes
Thomas Sicheritz-Ponten
 
O Thursday, September 17
14:00-14:45
Sequence comparisons, database searches, multiple alignments (slides)
Kristoffer Kiil
15:00-17:00
Exercise 5: BLAST, ClustalW, Muscle (slides)
Kristoffer Kiil
 
O Monday, September 21
13:00-13:45
Genome, Pan- and Metagenome assembly (slides)
Thomas Sicheritz-Ponten
14:00-17:00
Exercise: Assembly
Thomas Sicheritz-Ponten
 
O Thursday, September 24
13:00-13:45
Prediction of Gene Function and Cellular Localization
Ulrik Plate
14:00-17:00
Computer lab: Gene Function and Cellular Localization
Ulrik Plate
 
O Monday, September 28
13:00-13:45
The Pangenomic concept, variation within species, part I (Slides)
Dave Ussery
14:00-17:00
Exercise 6: Pangenomics, part I (Exercise)
Dave Ussery
 
O Thursday, October 1

Lecture and lab canceled due to illness

13:00-13:45
COG, KEGG, Gene Onthology, Enzyme Hunting and metabolic reconstructionsThomas Sicheritz-Ponten
14:00-17:00
Computer Lab: MG-Rast
Thomas Sicheritz-Ponten
 
O Monday, October 5
13:00-13:45
The Pangenomic concept, variation within species, part II (Slides/Notes)
Dave Ussery
14:00-17:00
Exercise 7: Pangenomics, part II (Exercise)
Dave Ussery
 
O Thursday, October 8
13:00-16:00
Computational Models of Microbial Communities
Dave Ussery
 
O Monday, October 12

Fall break
OThursday, October 15

Fall break
 
O Monday, October 19
13:00-13:45
Gene finding, Gene/Genome Annotation (Slides)
Carsten Friis
14:00-17:00
Exercise 8: Genome Annotation (Useful Links)
Carsten Friis
 
O Thursday, October 22
13:00-13:45
Microarrays and Transcriptomics in Metagenomics (slides)
Marcelo Bertalan
14:00-17:00
Computer Lab: Pangenomic and Metagenomic arrays (slides)
Marcelo Bertalan
 
O Monday, October 26
13:00-13:45
Student presentation of Genome Annotation Lab
Carsten Friis
14:00-
Initiation of Group Projects
Carsten Friis
 
O Thursday, October 29
13:00-13:45
Array-based Comparative Genomic Hybridization (aCGH) (Slides)
Carsten Friis
14:00-17:00
Exercise 9: aCGH Analysis (Useful Links)
Carsten Friis
 
O Monday, November 2
Deadline for Project Formulation
13:00-13:45
Presentations of Project Formulations
The Students
14:00-14:45
Recap on Pan-genomics (Papers)
Carsten Friis
15:00-17:00
Computer Lab: Optional exercise in Pan-genomics
Carsten Friis
 
O Thursday, November 5
13:00-14:00
Analysis of gene expression in infectious diseases (Slides)
Kirstine Klitgaard Schou
14:00-17:00
Project Work
 
O Monday, November 9
13:00-17:00
Project Work
 
O Thursday, November 12

Lecture canceled

13:00-17:00
Project Work on your own
 
O Monday, November 16
Class moved to the CBS Library (Building 208 room 027)
13:00-14:00
Guest Lecture
TBA
14:00-17:00
Project Work
 
O Thursday, November 19
Class moved to the CBS Library (Building 208 room 027)
13:00-14:00
Guest Lecture: DNA from the Polar Seas
Nikolaj Blom
14:00-17:00
Project Work
 
O Monday, November 23
13:00-14:00
Origin of replication and strand bias in bacterial genomes
Peder Worning
14:00-17:00
Project Work
 
O Thursday, November 26
Deadline for handing in Project Work Summary
13:00-14:00
Pangenomics of Staphylococcus aureus
Anja Stausgaard
14:00-17:00
Project Work
 
O Monday, November 30
Deadline for Project Work
13:00-?
Student Presentation of Project Work
The Student Project Groups
 
O Thursday, December 3
Exam preparation (2008 Exam)
 
O Thursday, December 10
The Exam!