INSTRUCTIONS for using the NetPlantGene mail server:

In order to use the mail server for prediction on nucleotide sequences: 


1) Prepare a text file including one or more sequences.  The  sequences
must be preceded by a line starting by the symbol > followed by a  name
(identifyer) of the sequence. There must be at least one  character  at
each line of each sequence. 

The sequences must be submitted using the one letter abbreviations  for
the nucleotides: `acgtACGT'. 
N.B. Other characters will be accepted, but not encoded in the  network
window, when making the prediction.  
	
Example: Create a text file: `sequence.txt' using an editor, the syntax
of the file may look like this: 
	
>seq_name1 example
TTCTCGTAGGTGGCATTCAATTTGCTACTGCTCAATTGCCCCTTCTTGAGCTGATGGATTGTGGCATG
ACTGTCTCTGATCCCAACTCAGATAATCCGACCTTTGTAGAGAATCCAAGTCCACATAAAACACCAGG
CTACAATCAAAAGATGTTTATCAAACATAAACGGCTGAAGAAACTTAGTTTGTGGGGATGTCTCCAGT
.
.
TTCTCGTAGGTGGCATTCAATTTGCTACTGCTCAATTGCCCCTTCTTGAGCTGATGGATTGTGGCATG
ACATAAACGGCTGAAGAAACTTAGTTTGTGGGGATGTCTCCAGTTTGGACGTGAGATTCCTC

>seq_name2 example
GCATCAGGATGTCAAGGCCTATTGACCGGAGCTATCCGAAAACAGGTAATCCCTTGAACATCTGTGAT
GTCTTAGACATGTGATTAGTAAAAAACAATCTGTTTGAGTCACACCCAACCCAAACTCTTAAGAAGCG
TCTCCTTATTTGTGATAGGTTTCTGAAAACTTCTCGGCTGGTGAGAACCATATGCCACGGAAACGCTT
.
.
GGCTGATGCATCTAAAAGGATTCAAGCTTTACCTTC 


2) Mail the text file to netplantgene@cbs.dtu.dk: 

In the UNIX environment you may mail the text  file  `sequence.txt'  to
netplantgene@cbs.dtu.dk by typing: 
	
mail netplantgene@cbs.dtu.dk < sequence.txt


3) You will receive a mail containing the prediction, or possibly error
messages from the server. If the file contains the  word  `help',  this
help file will be returned. Response time depends on system load.