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AMUSER - 1.0

AMUSER offers quick and easy design of PCR primers optimized for various USER cloning based DNA engineering

Instructions Output format Software download Article abstract

STEP 1: Input sequences

Paste in one or more DNA sequences in FASTA format

Perform DNA fusion, site directed mutagenesis, and combinatorial assembly of sequences. See how in the instructions.


(Click to enlarge overview)

or upload DNA sequences in FASTA format


Input is circular (only allows circular output)

STEP 2: Output construct

circular
linear
into a USER cassette (click to show list)

STEP 3: PCR experimental conditions

Default
Advanced




Restrictions:

Please read the CBS access policies for information about limitations on the daily number of submissions. The command-line version of AMUSER is free software, and be downloaded here: Software Download.

Confidentiality:
The sequences are kept confidential and will be deleted after processing.


CITATIONS

For publication of results, please cite:

Hans Jasper Genee, Mads Tvillinggaard Bonde, Frederik Otzen Bagger, Jakob Berg Jespersen, Morten O. A. Sommer, Rasmus Wernersson, and Lars Rønn Olsen
Software-supported USER cloning strategies for site-directed mutagenesis and DNA assembly

View the abstract.

Keywords: : DNA assembly, USER cloning, primer design, site-directed mutagenesis, point mutation, web server. -->


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