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Barracoda 1.0 Server

A tool for analysing DNA barcode sequencing data.

Andrea M. Marquard and Aron C. Eklund

Instructions Output format Article abstract


Sequencing data

Submit FASTA/FASTQ file:   

Barcode information

For each tag within the barcode, either submit a FASTA file of expected sequences, or paste the sequence if there is only one, or type the expected base pair length if the sequence is unknown.
Orientation: Sequences should be oriented according to the orientation of the individual A- and B-oligos before annealing. Ie., B-sequences should be the reverse complement of what is expected in the sequencing reads.

Sample identification tag     (select FASTA file)
Forward primer A (paste sequence)
N sequence (type length of tag)
Epitope tag A (select FASTA file)
Annealing region (paste sequence)
Epitope tag B (select FASTA file)
N sequence (type length of tag)
Forward primer B (paste sequence)

Sample identification

Upload a tab-delimited file of paired sequence IDs (1st column) and sample names (2nd column). The file should contain no headers.
The sequence IDs should be the same as those found in the Sample identification tag FASTA file uploaded above.
Leave sample name blank for unused sequence tags, and make sure to use identical names for replicates.

Sample identification table   

Barcode annotations

Upload a file with annotations for the barcodes.

Barcode annotations   


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