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Usage instructions
Submit...
A. by "cut and paste": (i) a single sequence in FASTA format or only
plain sequence, or (ii) multiple sequences in FASTA format.
B. a file in FASTA format. Type in the path to the file directly or use
the "Browse" button to find your file.
Include the N-terminus
It is strongly recommended to include the N-terminus of the submitted sequence.
The further from the N-terminal residue the submitted sequence starts, the more
difficult and unreliable the prediction will be.
Submit preferably around 100 residues
Submit if possible about 100 N-terminal residues. The suggested length is due
to the fact that the "cTP"/"no cTP" predictor was trained with input sequences
of length 100 residues. However, shorter sequences may also be satisfactory
predicted (it is more important that the N-terminal part is intact). The
cleavage site prediction is restricted to search for a potential cleavage site
within the 100 most N-terminal residues and is in itself not influenced by
sequence length.
Sequence names
The name of the sequences may be of any length, but only the first 20
characters will be preserved throughout the prediction and presented on the
ChloroP prediction result page.
Detailed output
Check this box if you would like the neural network score also for each
residue (and not only for each sequence) to be presented.
A derivative of the network score used for finding the area in which the
cleavage site is searched for (the 40 residues surrounding the residue with
the highest derivative score) is also presented, along with the cleavage site
score (CS-score) for each residue.
GETTING HELP
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