In order to use the DictyOglyc server for prediction on amino-acids
- Type the name of the sequence in the 'Sequence name' field
Click on the "Generate Graph" checkbox if you'd like to see a graph
with the output (Gif/Postscript). These may be quite useful in seeing the
"hot" spots in your protein.
Press the "Submit sequence" button.
A WWW page will return the results when the prediction is ready. Response
time depends on system load.
- The sequence must be submitted using the one letter code for the amino
- Other characters will be accepted, but not encoded in the network window,
when making the prediction. Do not use these.
- Peptides shorter than 19 residues can produce non-reliable results. (always
include 9 residues on both sides of the Ser/Thr you want evaluated. The
optimal is to use the complete "native" glycoprotein sequence truncating
the signal sequence)
Use the file browser to find your sequences in FASTA format in your own
appropiate directory (a single file may contain multiple sequences).
Example of two sequences in fasta format:
>seq1Click on Generate graph if required, and then press the "Send