EPipe 1.0 Server

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The EPipe 1.0 server performs comparative analysis of proteins derived from the same transcript by alternative splicing. The analysis is automated: the input proteins are first processed by a number of analysis and prediction methods, the results are then remapped onto a multiple alignment and compared. All the differences found between the isoforms are reported in detail. It is also possible to view the complete analysis and prediction results.

NOTE 1: This service is under development; new analysis and prediction methods are being added, the output format is being refined etc. As many external tools are called special attention is given to the overall speed of the service. Documentation is in preparation.

NOTE 2: EPipe is dependent on a number of other programs that have to be run on the input sequences prior to the comparative analysis. Therefore, the processing of multiple sequences may be time-consuming. In the case of prolonged wait the user is advised to use the e-mail option: at any time during the wait you may enter your e-mail address and simply leave the window. Your job will continue; you will be notified by e-mail when it has terminated. The e-mail message will contain the URL under which the results are stored; they will remain on the server for 24 hours for you to collect them.

Instructions Output format Article abstract

SUBMISSION

Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Alignment method

Alignment parameters T-COFFEE
gapopen = gapext=

CLUSTAL
gapopen = gapext= endgaps= gapdist=

Unspliced transcript
(not implemented yet)

Unspliced transcript in FASTA format if 'Align to unspliced transcript' was chosen above

Alignment
Custom alignment in FASTA if 'Custom alignment' was chosen above

Features to compare All features

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Module Description and URL Structural map

Restrictions:
At most ? sequences and ? amino acids per submission; each sequence not less than ? and not more than ? amino acids.

Confidentiality:
The sequences are kept confidential and will be deleted after processing.

EPipe 1.0 Server

SUBMISSION

GETTING HELP