Acidobacteria Actinobacteria Alphaproteobacteria
Aquificae Bacteroidetes/Chlorobi Betaproteobacteria
Chlamydiae/Verrucomicrobia Chloroflexi Cyanobacteria
Deinococcus-Thermus Deltaproteobacteria Epsilonproteobacteria
Firmicutes Fusobacteria Gammaproteobacteria
Other Bacteria Planctomycetes Spirochaetes
Thermotogae
Classification of phyla is from NCBI page
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Data for AE000512  

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hmmsearch - search a sequence database with a profile HMM
HMMER 2.3.2 (Oct 2003)
Copyright (C) 1992-2003 HHMI/Washington University School of Medicine
Freely distributed under the GNU General Public License (GPL)
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HMM file: /home/projects/MicrobialGenomicsGroup/Packages/hmmdata-1.0.0.package/Resources/noarch/TCS/HisKA_1.hmm [HisKA]
Sequence database: AE000512.Gprot.fsa
per-sequence score cutoff: >= 26.8 [TC1]
per-domain score cutoff: >= 26.8 [TC2]
per-sequence Eval cutoff: [none]
per-domain Eval cutoff: [none]
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Query HMM: HisKA
Accession: PF00512.12
Description: His Kinase A (phosphoacceptor) domain
[HMM has been calibrated; E-values are empirical estimates]

Scores for complete sequences (score includes all domains):
Sequence Description Score E-value N
-------- ----------- ----- ------- ---
AE000512_1627 CDS_1644628-1643390 97.6 1.2e-27 1
AE000512_0841 CDS_878315-879784 90.5 1.2e-25 1
AE000512_1336 CDS_1376583-1378850 60.0 5.1e-17 1
AE000512_0391 CDS_420106-421323 54.1 2.5e-15 1
AE000512_1239 CDS_1286306-1285347 41.6 8.8e-12 1

Parsed for domains:
Sequence Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
AE000512_1627 1/1 185 252 .. 1 87 [] 97.6 1.2e-27
AE000512_0841 1/1 250 318 .. 1 87 [] 90.5 1.2e-25
AE000512_1336 1/1 537 603 .. 1 87 [] 60.0 5.1e-17
AE000512_0391 1/1 192 258 .. 1 87 [] 54.1 2.5e-15
AE000512_1239 1/1 106 171 .. 1 87 [] 41.6 8.8e-12

Alignments of top-scoring domains:
AE000512_1627: domain 1 of 1, from 185 to 252: score 97.6, E = 1.2e-27
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+++ef+a++sHELRTPLt+I gyae+Lle ++l +++
AE000512_1 185 MRREFIATVSHELRTPLTSIHGYAETLLE---------DDLENKEL- 221

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
++++l++i++e+ r+ rLindlLdl++ie+
AE000512_1 222 ---------VKRFLKIIEEESARMTRLINDLLDLEKIEES 252

AE000512_0841: domain 1 of 1, from 250 to 318: score 90.5, E = 1.2e-25
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+k+ef+an+sHELRTPLtaI++yae++ + l +el+ ++
AE000512_0 250 MKTEFIANISHELRTPLTAIKAYAETIYNSL-------GELDLSTL- 288

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+e+le+i++++++l++L+n+lLd+sr+e
AE000512_0 289 ----------KEFLEVIIDQSNHLENLLNELLDFSRLERK 318

AE000512_1336: domain 1 of 1, from 537 to 603: score 60.0, E = 5.1e-17
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++e++a ++HE+R+P+t I g++ +++ + ++e++
AE000512_1 537 ILGEMTARVAHEIRNPITIIGGFIMRMKK---------HLDDPETL- 573

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
++y+++i +e+ rl+ ++ ++L++s+ ++
AE000512_1 574 ----------KKYINIITNELSRLETIVKEILEYSKERQV 603

AE000512_0391: domain 1 of 1, from 192 to 258: score 54.1, E = 2.5e-15
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++ +f ++sHEL+TPL+++++ +++L+ +++l+++
AE000512_0 192 SLKRFSMDASHELKTPLSVLKTSLDVLKY--------REDLPDDV-- 228

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+ l ++++ +e ++++in+lL l + e
AE000512_0 229 ----------KNKLGIMEKNVEKMSKQINQLLLLVKSEGS 258

AE000512_1239: domain 1 of 1, from 106 to 171: score 41.6, E = 8.8e-12
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
ak +f+ ++sHEL TPL+a ++++ lL++ e ++e+
AE000512_1 106 AKLDFVTAVSHELFTPLSASKANVFLLKD---------IENDPEK-- 141

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
e+l +++r+++r++ +i++l l+ i+
AE000512_1 142 ----------LEILGKVERSLDRMETIIRQLKVLTMIQLR 171


Histogram of all scores:
score obs exp (one = represents 6 sequences)
----- --- ---
-14 8 0|==
-13 26 1|*====
-12 88 42|======*========
-11 191 218|================================ *
-10 326 405|======================================================= *
-9 347 419|==========================================================*
-8 290 313|================================================= *
-7 206 197|================================*==
-6 126 113|==================*==
-5 99 62|==========*======
-4 53 33|=====*===
-3 37 17|==*====
-2 14 9|=*=
-1 11 4|*=
0 5 2|*
1 3 1|*
2 1 0|=
3 1 0|=
4 4 0|=
5 0 0|
6 4 0|=
> 7 6 -|=


% Statistical details of theoretical EVD fit:
mu = -8.9758
lambda = 0.6525
chi-sq statistic = 141.4013
P(chi-square) = 2.175e-25

Total sequences searched: 1846

Whole sequence top hits:
tophits_s report:
Total hits: 5
Satisfying E cutoff: 5
Total memory: 20K

Domain top hits:
tophits_s report:
Total hits: 5
Satisfying E cutoff: 5
Total memory: 22K


Acknowledgements:

This research is funded by:

Developed by: