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Thermotogae
Classification of phyla is from NCBI page
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Data for AM942444  

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hmmsearch - search a sequence database with a profile HMM
HMMER 2.3.2 (Oct 2003)
Copyright (C) 1992-2003 HHMI/Washington University School of Medicine
Freely distributed under the GNU General Public License (GPL)
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /home/projects/MicrobialGenomicsGroup/Packages/hmmdata-1.0.0.package/Resources/noarch/TCS/HisKA_1.hmm [HisKA]
Sequence database: AM942444.Gprot.fsa
per-sequence score cutoff: >= 26.8 [TC1]
per-domain score cutoff: >= 26.8 [TC2]
per-sequence Eval cutoff: [none]
per-domain Eval cutoff: [none]
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query HMM: HisKA
Accession: PF00512.12
Description: His Kinase A (phosphoacceptor) domain
[HMM has been calibrated; E-values are empirical estimates]

Scores for complete sequences (score includes all domains):
Sequence Description Score E-value N
-------- ----------- ----- ------- ---
AM942444_1634 CDS_1907040-1905451 75.5 2.4e-21 1
AM942444_0481 CDS_577230-579332 71.7 2.8e-20 1
AM942444_1441 CDS_1689591-1688425 61.2 2.6e-17 1
AM942444_0546 CDS_656212-657801 52.2 9.2e-15 1
AM942444_0642 CDS_763479-762445 40.1 2.5e-11 1

Parsed for domains:
Sequence Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
AM942444_1634 1/1 297 361 .. 1 87 [] 75.5 2.4e-21
AM942444_0481 1/1 389 456 .. 1 87 [] 71.7 2.8e-20
AM942444_1441 1/1 148 214 .. 1 87 [] 61.2 2.6e-17
AM942444_0546 1/1 284 356 .. 1 87 [] 52.2 9.2e-15
AM942444_0642 1/1 144 209 .. 1 87 [] 40.1 2.5e-11

Alignments of top-scoring domains:
AM942444_1634: domain 1 of 1, from 297 to 361: score 75.5, E = 2.4e-21
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+++f+ ++sHELRTPLt++rgyael + ++++
AM942444_1 297 TMRRFIGDASHELRTPLTSVRGYAELYRS----------GATDDA-- 331

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+ +i +eaer+ L++dlL l r+++g
AM942444_1 332 ----------DMVIGRISDEAERMGLLVEDLLALVRMDEG 361

AM942444_0481: domain 1 of 1, from 389 to 456: score 71.7, E = 2.8e-20
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+++f++++sHELRTPLt++r++a+l+++++ ++l++ +
AM942444_0 389 LQRQFTSDVSHELRTPLTTVRMAADLIKDNA-------ENLDPMT-- 426

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+++ e +++e++r + L+ dlL++sr +ag
AM942444_0 427 ----------ARAAELMDNELDRFEMLLGDLLEISRHDAG 456

AM942444_1441: domain 1 of 1, from 148 to 214: score 61.2, E = 2.6e-17
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+++f+an+sHEL+TP+ aI+++ae+ + +++ +e
AM942444_1 148 VRRDFVANVSHELKTPIGAISLLAEAVTD--------YSDDPEAV-- 184

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+++ +i++e+ rl +L++++ dls+ + +
AM942444_1 185 ----------HRFGGKIQKESTRLVHLVQEIIDLSQVQDN 214

AM942444_0546: domain 1 of 1, from 284 to 356: score 52.2, E = 9.2e-15
*->akseflanlsHELRTPLtaIrgyaelLleeldkv.lagdtelseeqr
+ e++a++sHEL+TPLt++r+++elLl + ++ +e +r
AM942444_0 284 KQKELVADASHELKTPLTSLRTNIELLLL----AtRENSPGITEADR 326

lpeLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+++ + +l++++ +++Li dl dl+r + +
AM942444_0 327 -----------QDLERDVLSQLDEMSALIKDLVDLAREDGP 356

AM942444_0642: domain 1 of 1, from 144 to 209: score 40.1, E = 2.5e-11
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++++f++n+sHEL+TP+++I+++a+++l+ +++ +
AM942444_0 144 RQRRFASNASHELKTPIATIQTMADVALA----------SGDAQVL- 179

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
r+ l ++++ r + + lL+l+ +
AM942444_0 180 ----------RDTLGRVREVNARAADTVASLLQLANVDVR 209


Histogram of all scores:
score obs exp (one = represents 8 sequences)
----- --- ---
-15 1 0|=
-14 8 0|=
-13 14 1|*=
-12 70 46|=====*===
-11 155 239|==================== *
-10 376 445|=============================================== *
-9 431 459|====================================================== *
-8 333 344|==========================================*
-7 248 216|==========================*====
-6 158 124|===============*====
-5 105 68|========*=====
-4 50 36|====*==
-3 37 19|==*==
-2 17 10|=*=
-1 4 5|*
0 5 2|*
1 3 1|*
2 1 0|=
3 2 0|=
> 4 6 -|=


% Statistical details of theoretical EVD fit:
mu = -8.9758
lambda = 0.6525
chi-sq statistic = 113.4573
P(chi-square) = 1.07e-19

Total sequences searched: 2024

Whole sequence top hits:
tophits_s report:
Total hits: 5
Satisfying E cutoff: 5
Total memory: 20K

Domain top hits:
tophits_s report:
Total hits: 5
Satisfying E cutoff: 5
Total memory: 22K


Acknowledgements:

This research is funded by:

Developed by: