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Firmicutes Fusobacteria Gammaproteobacteria
Other Bacteria Planctomycetes Spirochaetes
Thermotogae
Classification of phyla is from NCBI page
Search:
264 projects found  

1 accessions found for Escherichia coli BL21(DE3)  

Data for AM946981  

Available Atlases
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hmmsearch - search a sequence database with a profile HMM
HMMER 2.3.2 (Oct 2003)
Copyright (C) 1992-2003 HHMI/Washington University School of Medicine
Freely distributed under the GNU General Public License (GPL)
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /home/projects/MicrobialGenomicsGroup/Packages/hmmdata-1.0.0.package/Resources/noarch/TCS/HisKA_1.hmm [HisKA]
Sequence database: AM946981.Gprot.fsa
per-sequence score cutoff: >= 26.8 [TC1]
per-domain score cutoff: >= 26.8 [TC2]
per-sequence Eval cutoff: [none]
per-domain Eval cutoff: [none]
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query HMM: HisKA
Accession: PF00512.12
Description: His Kinase A (phosphoacceptor) domain
[HMM has been calibrated; E-values are empirical estimates]

Scores for complete sequences (score includes all domains):
Sequence Description Score E-value N
-------- ----------- ----- ------- ---
AM946981_2593 CDS_2746855-2749611 100.1 5.1e-28 1
AM946981_0352 CDS_383192-384487 91.0 2e-25 1
AM946981_2241 CDS_2361572-2365165 81.6 9.1e-23 1
AM946981_1003 CDS_1059876-1057132 80.7 1.6e-22 1
AM946981_2104 CDS_2209499-2206698 80.6 1.8e-22 1
AM946981_3026 CDS_3220732-3218396 78.9 5.5e-22 1
AM946981_2105 CDS_2209714-2211540 76.4 2.8e-21 1
AM946981_2412 CDS_2556113-2554686 75.4 5.3e-21 1
AM946981_1975 CDS_2061111-2062514 69.8 2.1e-19 1
AM946981_3746 CDS_4010976-4009603 66.6 1.6e-18 1
AM946981_4240 CDS_4552086-4553510 62.4 2.5e-17 1
AM946981_3833 CDS_4108060-4109325 62.1 3e-17 1
AM946981_0520 CDS_553252-551828 61.0 6.3e-17 1
AM946981_2848 CDS_3037125-3038474 60.7 7.9e-17 1
AM946981_3703 CDS_3961115-3960066 59.7 1.5e-16 1
AM946981_0643 CDS_683696-681012 59.6 1.6e-16 1
AM946981_1873 CDS_1942612-1941254 52.3 1.8e-14 1
AM946981_3944 CDS_4238441-4237350 45.0 2.2e-12 1
AM946981_1568 CDS_1627488-1628789 42.4 1.2e-11 1

Parsed for domains:
Sequence Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
AM946981_2593 1/1 292 357 .. 1 87 [] 100.1 5.1e-28
AM946981_0352 1/1 203 269 .. 1 87 [] 91.0 2e-25
AM946981_2241 1/1 711 777 .. 1 87 [] 81.6 9.1e-23
AM946981_1003 1/1 443 508 .. 1 87 [] 80.7 1.6e-22
AM946981_2104 1/1 453 518 .. 1 87 [] 80.6 1.8e-22
AM946981_3026 1/1 282 347 .. 1 87 [] 78.9 5.5e-22
AM946981_2105 1/1 388 453 .. 1 87 [] 76.4 2.8e-21
AM946981_2412 1/1 249 317 .. 1 87 [] 75.4 5.3e-21
AM946981_1975 1/1 240 304 .. 1 87 [] 69.8 2.1e-19
AM946981_3746 1/1 238 300 .. 1 87 [] 66.6 1.6e-18
AM946981_4240 1/1 255 320 .. 1 87 [] 62.4 2.5e-17
AM946981_3833 1/1 237 303 .. 1 87 [] 62.1 3e-17
AM946981_0520 1/1 261 327 .. 1 87 [] 61.0 6.3e-17
AM946981_2848 1/1 236 302 .. 1 87 [] 60.7 7.9e-17
AM946981_3703 1/1 129 186 .. 1 79 [. 59.7 1.5e-16
AM946981_0643 1/1 663 730 .. 1 87 [] 59.6 1.6e-16
AM946981_1873 1/1 235 301 .. 1 87 [] 52.3 1.8e-14
AM946981_3944 1/1 142 202 .. 1 87 [] 45.0 2.2e-12
AM946981_1568 1/1 211 271 .. 1 87 [] 42.4 1.2e-11

Alignments of top-scoring domains:
AM946981_2593: domain 1 of 1, from 292 to 357: score 100.1, E = 5.1e-28
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
kseflan+sHELRTPL++++g++ l l+ tel++ q
AM946981_2 292 IKSEFLANMSHELRTPLNGVIGFTRLTLK---------TELTPTQ-- 327

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
r+ l+ti+r+a+ ll++ind+Ld+s++eag
AM946981_2 328 ----------RDHLNTIERSANNLLAIINDVLDFSKLEAG 357

AM946981_0352: domain 1 of 1, from 203 to 269: score 91.0, E = 2e-25
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
a++ f+an+sHELRTPLt+++gy+e++ e ++l r
AM946981_0 203 ARRNFFANVSHELRTPLTVLQGYLEMMDE---------QPLEGAVR- 239

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+++l+t++++++r++ L+ +lL+ls+iea
AM946981_0 240 ----------EKALHTMREQTQRMEGLVKQLLTLSKIEAA 269

AM946981_2241: domain 1 of 1, from 711 to 777: score 81.6, E = 9.1e-23
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
aks+fla++sHE+RTP+++I+g++elL + ls eqr
AM946981_2 711 AKSQFLATMSHEIRTPISSIMGFLELLSG---------SGLSKEQR- 747

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
e+++ ++ +++ll Li ++Ld +ie+g
AM946981_2 748 ----------VEAISLAYATGQSLLGLIGEILDVDKIESG 777

AM946981_1003: domain 1 of 1, from 443 to 508: score 80.7, E = 1.6e-22
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
aks fla++sHE+RTPL +I+g+a+lL + ++ q
AM946981_1 443 AKSAFLAAMSHEIRTPLYGILGTAQLLAD---------NPALNAQ-- 478

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
r+ l+ i +++e+ll ++nd+Ld+s ieag
AM946981_1 479 ----------RDDLRAITDSGESLLTILNDILDYSAIEAG 508

AM946981_2104: domain 1 of 1, from 453 to 518: score 80.6, E = 1.8e-22
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+ks fla++sHELRTPL +I+g+++lL++ el+
AM946981_2 453 SKSMFLATVSHELRTPLYGIIGNLDLLQT---------KELPKGV-- 488

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+++ + +++ ll++i+d+Ld+s+ie++
AM946981_2 489 ----------DRLVTAMNNSSSLLLKIISDILDFSKIESE 518

AM946981_3026: domain 1 of 1, from 282 to 347: score 78.9, E = 5.5e-22
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
k+ f++++sHELRTPL++I g++ +Ll+ tel++eq
AM946981_3 282 DKTTFISTISHELRTPLNGIVGLSRILLD---------TELTAEQ-- 317

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
++yl+ti+ +a l ++ nd+ d+ ++e
AM946981_3 318 ----------EKYLKTIHVSAVTLGNIFNDIIDMDKMERR 347

AM946981_2105: domain 1 of 1, from 388 to 453: score 76.4, E = 2.8e-21
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++e++a+++HE R+PLtaIrgy+++L+ ++ ++ +
AM946981_2 388 TLGELMAGVAHEVRNPLTAIRGYVQILRQ---------QTSDPIH-- 423

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+eyl+++l+e++++++ i++lL++sr ++
AM946981_2 424 ----------QEYLSVVLKEIDSINKVIQQLLEFSRPRHS 453

AM946981_2412: domain 1 of 1, from 249 to 317: score 75.4, E = 5.3e-21
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++ +fl++lsHEL+TPL+++r +elL +++ ++l++eq
AM946981_2 249 QRHQFLRHLSHELKTPLASMREGTELLADQV------VGPLTPEQ-- 287

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+e +++++++++ l++Li++lLd+ r +a
AM946981_2 288 ----------KEVVSILDSSSRNLQKLIEQLLDYNRKQAD 317

AM946981_1975: domain 1 of 1, from 240 to 304: score 69.8, E = 2.1e-19
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++++f+a++sHELRTPL+++rg +e++++++ + ++e+
AM946981_1 240 MRRDFMADISHELRTPLAVLRGELEAIQDGV-------RKFTPET-- 277

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+ +++ e+ l +L++dl +ls ++g
AM946981_1 278 -------------VASLQAEVGTLTKLVDDLHQLSMSDEG 304

AM946981_3746: domain 1 of 1, from 238 to 300: score 66.6, E = 1.6e-18
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+++++l+++sHELRTPLt +++ + lL++ +++
AM946981_3 238 SQQRLLSDISHELRTPLTRLQLGTALLRR---------RSGESKE-- 273

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
le+i+ ea+rl+++indlL +sr ++
AM946981_3 274 -------------LERIETEAQRLDSMINDLLVMSRNQQK 300

AM946981_4240: domain 1 of 1, from 255 to 320: score 62.4, E = 2.5e-17
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++ + +l HEL+ PL+aIrg+ae+L+e ++ +e
AM946981_4 255 YIEQYVYALTHELKSPLAAIRGAAEILRE---------GPPPEVV-- 290

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+++++ il + r+++L++ lL +r+e+
AM946981_4 291 ----------ARFTDNILTQNARMQALVETLLRQARLENR 320

AM946981_3833: domain 1 of 1, from 237 to 303: score 62.1, E = 3e-17
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
a++ ++a+++HE+R+PL++I+g+a+ e +++++ e
AM946981_3 237 ALGHLAAGVAHEIRNPLSSIKGLAKYFAE--------RAPAGGEA-- 273

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+++ +++ +ea+rl+r +++lL+l r +
AM946981_3 274 ----------HQLAQVMAKEADRLNRVVSELLELVRPTHL 303

AM946981_0520: domain 1 of 1, from 261 to 327: score 61.0, E = 6.3e-17
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++s f a+++HE+RTP+t +++ +e++l + +s +++
AM946981_0 261 RQSNFSADIAHEIRTPITNLITQTEIALS---------QSRSQKEL- 297

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
++ l + l+e+ r+ ++++d+L l++++++
AM946981_0 298 ----------EDVLYSNLEELTRMAKMVSDMLFLAQADNN 327

AM946981_2848: domain 1 of 1, from 236 to 302: score 60.7, E = 7.9e-17
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+ ++f+++++HELR PLta++ +e+++ ++ +++ r
AM946981_2 236 RERRFTSDAAHELRSPLTALKVQTEVAQL---------SDDDPQAR- 272

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+++l +++ ++r rL+++lL+lsr+++
AM946981_2 273 ----------KKALLQLHSGIDRATRLVDQLLTLSRLDSL 302

AM946981_3703: domain 1 of 1, from 129 to 186: score 59.7, E = 1.5e-16
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
a ++++++l+HE+++PL ++rg+a+lL + +l+++ +
AM946981_3 129 AARDLVRGLAHEIKNPLGGLRGAAQLLSK----------ALPDPSL- 164

peLRledpeyreyletilreaerllrLindlL<-*
ey+++i+++a+rl++L+++lL
AM946981_3 165 ----------LEYTKVIIEQADRLRNLVDRLL 186

AM946981_0643: domain 1 of 1, from 663 to 730: score 59.6, E = 1.6e-16
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++ +la+lsH+LRTPLt++ g ae+L +l + ++++
AM946981_0 663 IRNALLAALSHDLRTPLTVLFGQAEILTLDL-------ASEGSPH-- 700

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
++ + i++ + rL+n+lLd++ri++g
AM946981_0 701 ----------ARQASEIRQHVLNTTRLVNNLLDMARIQSG 730

AM946981_1873: domain 1 of 1, from 235 to 301: score 52.3, E = 1.8e-14
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++s+f+ +l+HELRTP++a++g ++ l +++s +
AM946981_1 235 RLSQFADDLAHELRTPINALLGQNQVTLS---------QTRSIAE-- 270

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
y++ + ++e+e ++rL +++L l+r++ +
AM946981_1 271 ---------YQKTIAGNIEELENISRLTENILFLARADKN 301

AM946981_3944: domain 1 of 1, from 142 to 202: score 45.0, E = 2.2e-12
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+ f+a+++HELRTPL+++r+++elL + t+
AM946981_3 142 NERLFTADVAHELRTPLAGVRLHLELLAK---------TH------- 172

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+ + + ++++ + +++lL+l+r+++
AM946981_3 173 ----------HIDVAPLVARLDQMMESVSQLLQLARAGQS 202

AM946981_1568: domain 1 of 1, from 211 to 271: score 42.4, E = 1.2e-11
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+k +++ +++HELRTPL +r +e+ + ls+ +
AM946981_1 211 SKKQLIDGIAHELRTPLVRLRYRLEMSDN-----------LSAAE-- 244

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+ + r++ +l++Li++lL+++r++ +
AM946981_1 245 -------------SQALNRDISQLEALIEELLTYARLDRP 271


Histogram of all scores:
score obs exp (one = represents 14 sequences)
----- --- ---
-17 1 0|=
-16 1 0|=
-15 2 0|=
-14 10 0|=
-13 51 3|*===
-12 185 93|======*=======
-11 433 484|=============================== *
-10 693 898|================================================== *
-9 779 928|======================================================== *
-8 695 694|=================================================*
-7 502 437|===============================*====
-6 296 252|=================*====
-5 191 138|=========*====
-4 84 73|=====*
-3 50 39|==*=
-2 26 20|=*
-1 34 10|*==
0 15 5|*=
1 7 2|*
2 9 1|*
3 2 0|=
4 0 0|
> 5 21 -|==


% Statistical details of theoretical EVD fit:
mu = -8.9758
lambda = 0.6525
chi-sq statistic = 275.7580
P(chi-square) = 0

Total sequences searched: 4244

Whole sequence top hits:
tophits_s report:
Total hits: 19
Satisfying E cutoff: 19
Total memory: 21K

Domain top hits:
tophits_s report:
Total hits: 19
Satisfying E cutoff: 19
Total memory: 28K


Acknowledgements:

This research is funded by:

Developed by: