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1 accessions found for Methanospirillum hungatei JF-1  

Data for CP000254  

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hmmsearch - search a sequence database with a profile HMM
HMMER 2.3.2 (Oct 2003)
Copyright (C) 1992-2003 HHMI/Washington University School of Medicine
Freely distributed under the GNU General Public License (GPL)
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HMM file: /home/projects/MicrobialGenomicsGroup/Packages/hmmdata-1.0.0.package/Resources/noarch/TCS/HisKA_1.hmm [HisKA]
Sequence database: CP000254.Gprot.fsa
per-sequence score cutoff: >= 26.8 [TC1]
per-domain score cutoff: >= 26.8 [TC2]
per-sequence Eval cutoff: [none]
per-domain Eval cutoff: [none]
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Query HMM: HisKA
Accession: PF00512.12
Description: His Kinase A (phosphoacceptor) domain
[HMM has been calibrated; E-values are empirical estimates]

Scores for complete sequences (score includes all domains):
Sequence Description Score E-value N
-------- ----------- ----- ------- ---
CP000254_2603 CDS_2962024-2963964 89.6 3.6e-25 1
CP000254_2556 CDS_2913373-2914566 71.9 3.9e-20 1
CP000254_2787 CDS_3157582-3160374 52.2 1.5e-14 1
CP000254_1952 CDS_2283148-2281613 50.0 6.1e-14 1
CP000254_2945 CDS_3310713-3311780 49.9 6.6e-14 1
CP000254_1389 CDS_1657806-1656268 48.6 1.5e-13 1
CP000254_0854 CDS_990238-992730 41.1 2.1e-11 1
CP000254_1732 CDS_2027541-2024587 40.0 4.1e-11 1
CP000254_1396 CDS_1665338-1662993 39.3 6.4e-11 1
CP000254_1369 CDS_1624887-1618669 34.7 1.4e-09 2
CP000254_0155 CDS_177343-179532 34.4 1.6e-09 1
CP000254_0956 CDS_1109038-1107683 33.1 3.7e-09 1
CP000254_1313 CDS_1557675-1553845 31.8 9.1e-09 2
CP000254_3103 CDS_3496621-3495446 29.8 3.2e-08 1
CP000254_0005 CDS_5172-9371 29.3 4.4e-08 1
CP000254_1371 CDS_1629664-1626497 28.7 6.6e-08 1

Parsed for domains:
Sequence Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
CP000254_2603 1/1 418 486 .. 1 87 [] 89.6 3.6e-25
CP000254_2556 1/1 153 221 .. 1 87 [] 71.9 3.9e-20
CP000254_2787 1/1 705 773 .. 1 87 [] 52.2 1.5e-14
CP000254_1952 1/1 289 354 .. 1 87 [] 50.0 6.1e-14
CP000254_2945 1/1 133 201 .. 1 87 [] 49.9 6.6e-14
CP000254_1389 1/1 283 348 .. 1 87 [] 48.6 1.5e-13
CP000254_0854 1/1 583 648 .. 1 87 [] 41.1 2.1e-11
CP000254_1732 1/1 755 820 .. 1 87 [] 40.0 4.1e-11
CP000254_1396 1/1 552 616 .. 1 87 [] 39.3 6.4e-11
CP000254_0155 1/1 503 568 .. 1 87 [] 34.4 1.6e-09
CP000254_0956 1/1 214 279 .. 1 87 [] 33.1 3.7e-09
CP000254_3103 1/1 172 248 .. 1 87 [] 29.8 3.2e-08
CP000254_0005 1/1 440 485 .. 13 87 .] 29.3 4.4e-08
CP000254_1371 1/1 378 435 .. 1 87 [] 28.7 6.6e-08

Alignments of top-scoring domains:
CP000254_2603: domain 1 of 1, from 418 to 486: score 89.6, E = 3.6e-25
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
ks fla++sHELRTPL++I+g++ ++l +l ++l+ eq
CP000254_2 418 LKSAFLATMSHELRTPLNSIIGFTGIILQGLA------GPLNKEQ-- 456

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
++ l ++++a++ll+Lind+Ld+s+ieag
CP000254_2 457 ----------EKQLGMVQDSARHLLALINDVLDISKIEAG 486

CP000254_2556: domain 1 of 1, from 153 to 221: score 71.9, E = 3.9e-20
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+k++fl +++H+LR PL+ I+ y+e L e+l ++ls+e+
CP000254_2 153 MKNQFLGMAAHDLRSPLSLILNYTEFLMEDLQ------DNLSDEH-- 191

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
re+l+ti+ +a + + i d+Ld s ie+g
CP000254_2 192 ----------REFLQTIITSALDMKQVIADFLDVSIIESG 221

CP000254_2787: domain 1 of 1, from 705 to 773: score 52.2, E = 1.5e-14
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+++ f+ ++sH+LR P+ aI g+++lLl ++ ++ +e++
CP000254_2 705 ELDAFAYSVSHDLRGPVRAIDGFSHLLLLKA-------GDILDEEN- 743

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+ ++++i++++ ++rLi+dlL +sr +
CP000254_2 744 ----------KHLISRIRESVTTMDRLIQDLLRFSRTARQ 773

CP000254_1952: domain 1 of 1, from 289 to 354: score 50.0, E = 6.1e-14
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+k+ ++ +++H+LRTPLt +++++ lL++ ++ + +
CP000254_1 289 MKNLLITAIAHDLRTPLTPLIALLPLLEN---------EDDTQKR-- 324

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
e+++++++ a r+ ++i+ lL + ++++
CP000254_1 325 ----------TEIIQILEKNATRIASIIQTLLHFNDLGSP 354

CP000254_2945: domain 1 of 1, from 133 to 201: score 49.9, E = 6.6e-14
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+++ f ++sH+LR PL aI g++ +L+e + + l+++
CP000254_2 133 ELDSFSYSVSHDLRAPLIAIDGFSRILEETYA------GYLPPDG-- 171

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
e l++i+++a+++++Li+d+L lsr
CP000254_2 172 ----------VECLSRIRQAANQMDQLIEDFLRLSRVTRA 201

CP000254_1389: domain 1 of 1, from 283 to 348: score 48.6, E = 1.5e-13
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+k++f+ l+H+LRTPLt + + + l +e + +++
CP000254_1 283 QKDQFIYQLAHDLRTPLTPMVAILPLVKE---------AVSDPDS-- 318

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+ +l+ ++++++++++++d+++l r+ +
CP000254_1 319 ----------QSLLDMFQKSLRYMEQMVEDIMELIRLNSH 348

CP000254_0854: domain 1 of 1, from 583 to 648: score 41.1, E = 2.1e-11
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+k+ef+ + H+L+TPLt + + + +++ + +++++
CP000254_0 583 QKDEFIQIIGHDLKTPLTPLVAILPYIRK----------KVTDPEL- 618

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
e+l+ ++++ +++L+ +Ldl+++ +
CP000254_0 619 ----------CELLDNTIEDVSTIRKLVTTILDLAQMNSL 648

CP000254_1732: domain 1 of 1, from 755 to 820: score 40.0, E = 4.1e-11
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+k++f+ l H+L+TPLt +++++ +Ll +e ++e+
CP000254_1 755 QKDDFINQLGHDLKTPLTPLIALLPTLLH---------EEHDSEK-- 790

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
re+ ++lr++ +++++ + +L+l+r+
CP000254_1 791 ----------RELYGVLLRSVYSIREQTEKILTLARLSRH 820

CP000254_1396: domain 1 of 1, from 552 to 616: score 39.3, E = 6.4e-11
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+k++f+ l H+LRTPLt I+g++ L e l+ e++
CP000254_1 552 QKNDFIMQLGHDLRTPLTPILGLLPRLDE-----------LDGEEH- 586

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+ ++++i+r a++++++ + L l+r+ +
CP000254_1 587 ----------ANMVRIIERNARHIQNIASKSLKLARLNSL 616

CP000254_0155: domain 1 of 1, from 503 to 568: score 34.4, E = 1.6e-09
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+k+ef+ + H+L+T Lt +++ + +L++ e +++++
CP000254_0 503 QKDEFITQIGHDLKTSLTPVIAILPVLRR---------KESDPKKI- 539

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+yl+ ++r a ++ +L+ +L+++r+
CP000254_0 540 -----------QYLDMAIRNAAQMHDLLISILQMARLNKS 568

CP000254_0956: domain 1 of 1, from 214 to 279: score 33.1, E = 3.7e-09
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
++++++ + H+L+TPLt + +++ L e +ee+
CP000254_0 214 QRTDLITRIGHDLKTPLTPLYAFLPYLYQ---------KESDEEK-- 249

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+e+l+ + +++ l +L++++L ls i
CP000254_0 250 ----------KEILSLLVKDVTVLKHLVDRILKLSHITLD 279

CP000254_3103: domain 1 of 1, from 172 to 248: score 29.8, E = 3.2e-08
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+e++ ++ H++R+PL +I + lL++++ +++s e++
CP000254_3 172 VIGELATMIGHDMRNPLQVITNMQYLLEMRM-------QKMSKEELE 211

peLR..ledpeyreyletilreaerllrLindlLdlsrieag<-*
R++ e++++i e+++l+++i+dl d++r ++
CP000254_3 212 ILHRngI-----PEIFDRIGTEIQYLNKIISDLQDYAREVNP 248

CP000254_0005: domain 1 of 1, from 440 to 485: score 29.3, E = 4.4e-08
*->LRTPLtaIrgyaelLleeldkvlagdtelseeqrlpeLRledpeyre
+R+PLt I +++e++ + +
CP000254_0 440 IRNPLTIIATFTEMAAD----------------------------TQ 458

yletilreaerllrLindlLdlsrieag<-*
+ il e+ r+++++n Ld ++i ++
CP000254_0 459 TADLILAEISRIDEMVNS-LDREWIKSE 485

CP000254_1371: domain 1 of 1, from 378 to 435: score 28.7, E = 6.6e-08
*->akseflanlsHELRTPLtaIrgyaelLleeldkvlagdtelseeqrl
+ la l +R+PLt I+ ya++ ++
CP000254_1 378 ENIAQLAILNDGIRNPLTLISIYADMSED------------------ 406

peLRledpeyreyletilreaerllrLindlLdlsrieag<-*
+++ i++e++r+++++ + Ld ++ +g
CP000254_1 407 ----------VRITAQIQNEVKRIDEMVTN-LDREWVHSG 435


Histogram of all scores:
score obs exp (one = represents 9 sequences)
----- --- ---
-14 6 0|=
-13 60 2|*======
-12 190 71|=======*==============
-11 416 372|=========================================*=====
-10 531 690|==========================================================*
-9 526 712|==========================================================*
-8 492 533|======================================================= *
-7 335 336|=====================================*
-6 199 193|=====================*=
-5 148 106|===========*=====
-4 75 56|======*==
-3 38 30|===*=
-2 19 15|=*=
-1 19 8|*==
0 9 4|*
1 7 2|*
2 7 1|*
3 5 0|=
4 1 0|=
5 2 0|=
6 2 0|=
7 4 0|=
8 5 0|=
9 3 0|=
10 3 0|=
11 3 0|=
12 1 0|=
13 3 0|=
14 2 0|=
15 2 0|=
16 1 0|=
17 2 0|=
18 0 0|
19 2 0|=
20 4 0|=
21 0 0|
> 22 17 -|==


% Statistical details of theoretical EVD fit:
mu = -8.9758
lambda = 0.6525
chi-sq statistic = 328.3938
P(chi-square) = 0

Total sequences searched: 3139

Whole sequence top hits:
tophits_s report:
Total hits: 16
Satisfying E cutoff: 16
Total memory: 21K

Domain top hits:
tophits_s report:
Total hits: 14
Satisfying E cutoff: 14
Total memory: 26K


Acknowledgements:

This research is funded by:

Developed by: