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Supplementary Section for "Genomic Characterization of Campylobacter jejuni strain M1"

Carsten Friis, Trudy M. Wassenaar, Muhammad A. Javed, Lars Snipen, Karin Lagersen, Peter F. Hallin, Diane G. Newell, Monique Toszeghy, Anne Ridley, Georgina Manning and David W. Ussery


Abstract

Campylobacter jejuni strain M1 (laboratory designation 99/308) is a rarely documented case of direct transmission of C. jejuni from chicken to a person, resulting in enteritis. We have sequenced the genome of C. jejuni strain M1, and compared this to 12 other C. jejuni sequenced genomes currently publically available. Compared to these, M1 is closest to strain 81116. Based on the 13 genome sequences, we have identified the C. jejuni pan-genome, as well as the core genome, the auxiliary genes, and genes unique between strains M1 and 81116. The pan-genome contains 2,427 gene families, whilst the core genome comprised 1,295 gene families, or about two-thirds of the gene content of the average of the sequenced C. jejuni genomes. Various comparison and visualization tools were applied to the 13 C. jejuni genome sequences, including a species pan- and core genome plot, a BLAST Matrix and a BLAST Atlas. Trees based on 16S rRNA sequences and on the total gene families in each genome are presented. The findings are discussed in the background of the proven virulence potential of M1.



Supplementary Table 1
The table gives the positions of the 85 genes for which the position of the start codon was adjusted after comparison with NCBI nr.

Supplementary Figure 1
The figure shows the length distribution of the entire proteome of strain M1 as well as the sub-sets having matches in the SwissProt database or not. Since the length distribution should not diverge too much whether the proteins have homologs in Swissprot or not, the degree of overlap between the curves is an indication of the specificity of the gene finding.

Zommable Genome Atlas
This figure is an interactive version of the Genome Atlas from the paper. It is otherwise identical.

Zoomable BLAST Atlas
Like the Genome Atlas above, this is an interactive version of the BLAST Atlas from the paper. It includes additional annotation information not visible on the printed figure.