Usage instructions



1. Specify the input sequences

All the input sequences must be in one-letter amino acid code. The allowed alphabet (not case sensitive) is as follows:

A C D E F G H I K L M N P Q R S T V W Y and X (unknown)

All the other symbols will be converted to X before processing.

The sequences can be input in the following two ways:

Both ways can be employed at the same time: all the specified sequences will be processed.


2. Customize your run

Select the allele(s) you want to make predictions for from the scroll-down menu (select multiple alleles using the ctrl key), or type in the allele names separated by commas (with out blank spaces).

Define the length of the peptides to be analysed. Selection of multiple lengths possible.

Define the sorting mode

Define the sorting method

Give threshold value for binding values to be displayed.




3. Submit the job

Click on the "Submit" button. The status of your job (either 'queued' or 'running') will be displayed and constantly updated until it terminates and the server output appears in the browser window.

At any time during the wait you may enter your e-mail address and simply leave the window. Your job will continue; you will be notified by e-mail when it has terminated. The e-mail message will contain the URL under which the results are stored; they will remain on the server for 24 hours for you to collect them.