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Usage instructions



1. Specify the input sequences

All the input sequences must be in one-letter amino acid code. The allowed alphabet (not case sensitive) is as follows:

A C D E F G H I K L M N P Q R S T V W Y B Z X U

The sequences can be input in the following two ways:

  • Paste a single sequence (just the amino acids) or a number of sequences in FASTA format into the upper sequence window of the main server page.

  • Select a FASTA file on your local disk, either by typing the file name into the lower sequence window or by browsing the disk.

2. Specify the input variant data

All the input variant data must be in one-letter amino acid code. The allowed alphabet for the native and variant amino acids (not case sensitive) are as follows:

A C D E F G H I K L M N P Q R S T V W Y U

The format of the variant data has to be in the form:

'Accession' 'Native amino acid' 'Position' 'Variant amino acid'

where the accession has to correspond to the sequence identifier in the fasta sequence.

Example:
Accesssion S 2 T
Accesssion H 6 K
.
.

The variant data can be input in the following two ways:

  • Paste variant data into the upper variant data window of the main server page.

  • Select a variant file on your local disk, either by typing the file name into the lower variant data window or by browsing the disk.

3. Lowercase letters

All lowercase letters for amino acids in sequence and variant data will be changed to uppercase letters.


4. Submit the job

Click on the "Submit" button. The status of your job (either 'queued' or 'running') will be displayed and constantly updated until it terminates and the server output appears in the browser window.

At any time during the wait you may enter your e-mail address and simply leave the window. Your job will continue; you will be notified by e-mail when it has terminated. The e-mail message will contain the URL under which the results are stored; they will remain on the server for 24 hours for you to collect them.




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