1. Specify the input sequences
All the input sequences must be in one-letter amino acid
code. The allowed alphabet (not case sensitive) is as follows:
A C D E F G H I K L M N P Q R S T V W Y and X (unknown)
All the other symbols will be converted to X before processing. The
sequences can be input in the following two ways:
Paste a single sequence (just the amino acids) or a number of sequences
into the upper window of the main server page.
The sequence(s) should be either in FASTA
format or a list of peptides, one peptide pr. line all equal in length. If the input is in peptide format the peptide input checkbox should be checked.
file on your local disk, either by typing the file name into the lower window
or by browsing the disk.
The file must be in either FASTA
or peptide format as described above.
If the input is in peptide format the peptide input checkbox should be checked.
At most 5000 sequences per submission; each sequence not more than 20,000 amino acids and not less than 8 amino acids.
2. Customize your run
Select one or more of the alleles you want to make predictions for.
In the top of the list is the alleles for which available ANN predictors
are available (most reliable),
and in the bottom of the list are the alleles for which only PSSM predictors
are available (less relaiable).
In the allele selection window
it is indicated which allele is recommended to represent each supertype. This is
indicated as HLA-A*0101 (A1) neural network
for the A1 supertype for instance.
3. Submit the job
Click on the "Submit"
button. The status of your job (either 'queued'
or 'running') will be displayed and constantly updated until it terminates and
the server output appears in the browser window.
4. The Output
For a description of the output click here
At any time during the wait you may enter your e-mail address and simply leave
the window. Your job will continue; you will be notified by e-mail when it has
terminated. The e-mail message will contain the URL under which the results are
stored; they will remain on the server for 24 hours for you to collect them.