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NetMHCII 1.0 Server

NetMHCII 1.0 server predicts binding of peptides to a number of different HLA-DR alleles using position specific weight matrices (PSSM).

Predictions can be obtained for 14 HLA-DR alleles covering the 9 HLA-DR supertypes, and three mouse H2-IA alleles.

The prediction values are given in nM IC50 values, and strong and weak binding peptides are indicated in the output.

 

 

 

Instructions Output format Article abstract Data

SUBMISSION

Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

MHC Allele (use ctrl key to select multiple alleles)         Threshold         Peptide length         Sort by affinity 

Restrictions:
At most 5000 sequences per submission; each sequence not more than 20,000 amino acids and not less than 9 amino acids.

Confidentiality:
The sequences are kept confidential and will be deleted after processing.


CITATIONS

For publication of results, please cite:

Prediction of MHC class II binding affinity using SMM-align, a novel stabilization matrix alignment method.
Morten Nielsen*, Claus Lundegaard, and Ole Lund.
BMC Bioinformatics: 8: 238, 2007.
View the abstract, the full text version at BMC Bioinformatics: Full text

NOTE! The published version of the manuscript has an inconsistent reference listing. For a correct reference listing click here


PORTABLE VERSION

Would you prefer to run ...NetMHCII at your own site? NetMHCII v. 1.0 is available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at software@cbs.dtu.dk.


GETTING HELP

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