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Output format



DESCRIPTION

The prediction output consists of 10 columns.

  • Residue number (starting from 0)
  • HLA Allele
  • Peptide sequence
  • Protein identifier
  • Start position of binding core (starting from 0)
  • Binding core
  • Prediction score (1-log15K(aff))
  • Affinity IC50 value in nM
  • %Rank score - Percential rank for score to a set of 200,000 random natural peptides
  • Binding level, WB for weak biding, SB for strong binder



  • EXAMPLE OUTPUT

    
    
    # Input is in FSA format
    # Threshold for Strong binding peptides  50.000
    # Threshold for Weak binding peptides 500.000
    -----------------------------------------------------------------------------------------------------------------
      pos       HLA            peptide        Identity Pos       Core 1-log50k(aff) Affinity(nM)    %Rank  BindLevel
    -----------------------------------------------------------------------------------------------------------------
        0 DRB1*0401    ASQKRPSQRHGSKYL seq2_optional_c   6  SQRHGSKYL         0.041     10132.07    50.00
        1 DRB1*0401    SQKRPSQRHGSKYLA seq2_optional_c   5  SQRHGSKYL         0.060      8394.55    50.00
        2 DRB1*0401    QKRPSQRHGSKYLAT seq2_optional_c   4  SQRHGSKYL         0.077      7144.65    50.00
        3 DRB1*0401    KRPSQRHGSKYLATA seq2_optional_c   4  QRHGSKYLA         0.098      5824.18    50.00
        4 DRB1*0401    RPSQRHGSKYLATAS seq2_optional_c   3  QRHGSKYLA         0.121      4663.47    50.00
        5 DRB1*0401    PSQRHGSKYLATAST seq2_optional_c   6  SKYLATAST         0.167      3012.29    50.00
        6 DRB1*0401    SQRHGSKYLATASTM seq2_optional_c   6  KYLATASTM         0.324       665.84    32.00
        7 DRB1*0401    QRHGSKYLATASTMD seq2_optional_c   6  YLATASTMD         0.476       153.62     8.00 <= WB
        8 DRB1*0401    RHGSKYLATASTMDH seq2_optional_c   5  YLATASTMD         0.595        48.95     2.00 <= SB
        9 DRB1*0401    HGSKYLATASTMDHA seq2_optional_c   4  YLATASTMD         0.695        18.83     0.40 <= SB
       10 DRB1*0401    GSKYLATASTMDHAR seq2_optional_c   3  YLATASTMD         0.740        12.24     0.15 <= SB
       11 DRB1*0401    SKYLATASTMDHARH seq2_optional_c   2  YLATASTMD         0.722        14.55     0.20 <= SB
       12 DRB1*0401    KYLATASTMDHARHG seq2_optional_c   1  YLATASTMD         0.663        25.44     0.70 <= SB
       13 DRB1*0401    YLATASTMDHARHGF seq2_optional_c   0  YLATASTMD         0.480       148.99     7.00 <= WB
       14 DRB1*0401    LATASTMDHARHGFL seq2_optional_c   0  LATASTMDH         0.225      1727.75    50.00
    
    



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