The prediction output for each molecule consists of the following columns:
Seq Residue number (starting from 0)
Allele MHC molecule name
Peptide Amino acid sequence
Identity Annotation of the input sequence, if specified
Pos Starting position of the optimal binding core (starting from 0)
Core Binding core register
Core_Rel Reliability of the binding core, expressed as the fraction of networks in the ensemble selecting the optimal core (see the Core_Histograms for the reliability values of all cores)
1-log50K(aff) Predicted binding affinity in log-scale
Affinity(nM) Predicted binding affinity in nanomolar IC50
%Rank - % Rank of predicted affinity compared to a set of 200.000 random natural peptides. This measure is not affected by inherent bias of certain molecules towards higher or lower mean predicted affinities
Exp_bind If the input was given in PEPTIDE format with an annotated affinity value (mainly for benchmarking purposes).
BindingLevel (SB: strong binder, WB: weak binder). The peptide will be identified as a strong binder if the % Rank OR binding affinity (IC50) is below the specified threshold for the strong binders. The peptide will be identified as a weak binder if the % Rank OR binding affinity (IC50) is above the threshold of the strong binders but below the specified threshold for the weak binders.
If the "Graphical representation of the binding registers" option was selected, a list of figure links will be displayed. For the top 20 peptides, these graphs show the number of networks in the ensemble that agreed on each binding register (the Core_Rel measure for all registers). If the histogram shows alternative binding registers with comparable reliability measure (= similar height of the bars) for a predicted binder, that may suggest the presence of competitive binding cores within the peptide.