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NetMHCphosPan 1.0

The NetMHCphosPan-1.0 server predicts binding of phosphorylated peptides to any MHC molecule of known sequence using artificial neural networks (ANNs).

The server returns as default the likelihood of a peptide being a natural ligand of the selected MHC(s).

View the version history of this server. All previous versions are available online, for comparison and reference.


Instructions Output format Motif viewer Article abstract GitHub repo: Training and Evaluation Data sets

SUBMISSION

Hover the mouse cursor over the symbol for a short description of the options

INPUT TYPE:

Paste a single sequence or several sequences in FASTA format into the field below:

... or upload a file in FASTA format directly from your local disk:

... or load some sample data:



PEPTIDE LENGTH:  

You may select multiple lengths


SELECT SPECIES/LOCI:



Select Allele(s) (max 20 per submission)


... or type Allele names (i.e. HLA-A01:01) separated by commas and without spaces (max 20 per submission): 

For a list of allowed allele names click here

... or paste a single full length MHC protein sequence in FASTA format into the field below:

... or load a file containing a full length MHC protein sequence in FASTA format directly from your local disk:



ADDITIONAL CONFIGURATION:

Threshold for strong binder: % Rank 

Threshold for weak binder: % Rank 

Filtering threshold for %Rank (leave -99 to print all) 

Sort by prediction score 

Save predictions to XLS file 





Restrictions:
At most 5000 sequences per submission; each sequence not more than 20,000 amino acids and not less than 8 amino acids. Max 20 MHC alleles per submission.

Confidentiality:
The sequences are kept confidential and will be deleted after processing.


CITATIONS

For publication of results, please cite:

  • This version:

DATA RESOURCES

Data resources used to develop this server was obtained from

  • IEDB database.
    • Quantitative peptide binding data were obtained from the IEDB database.
  • IMGT/HLA database. Robinson J, Malik A, Parham P, Bodmer JG, Marsh SGE: IMGT/HLA - a sequence database for the human major histocompatibility complex. Tissue Antigens (2000), 55:280-287.
    • HLA protein sequences were obtained from the IMGT/HLA database (version 3.1.0).

PORTABLE VERSION

NetMHCphosPan v. 1.0 will be available as a stand-alone software package soon


GETTING HELP

Scientific problems:        Technical problems: