1. Specify the input sequencesAll the input sequences must be in one-letter amino acid code. The allowed alphabet (not case sensitive) is as follows:
All the other symbols will be converted to X before processing.
Note that for Peptide input, all peptides MUST of equal length. Note also, that you must click the box Click if input is PEPTIDE format if the input is in peptide format.
The sequences can be input in the following two ways:
Both ways can be employed at the same time: all the specified sequences will
be processed. However, there may be not more than 10 sequences
in total in one submission.
2. Customize your run
Select the allele(s) you want to make predictions for from the scroll-down menu (select multiple alleles using the
ctrl key), or type in the allele names separated by commas (with out blank spaces).
Give threshold value for binding values to be displayed.
Select if you want to include affinity predictions (Calculated with netMHCpan-2.8), and if this is the case, its contribution to the combined score.
Select one option from Sort by score to have the output sorted by descending order.
Click the box save prediction to xls file to save the raw prediction output to an excel file. This file
will be available in the bottom of the results output file.
3. Submit the jobClick on the "Submit" button. The status of your job (either 'queued' or 'running') will be displayed and constantly updated until it terminates and the server output appears in the browser window.
At any time during the wait you may enter your e-mail address and simply leave
the window. Your job will continue; you will be notified by e-mail when it has
Scientific problems: Technical problems: