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NetNGlyc 1.0 Server

The NetNglyc server predicts N-Glycosylation sites in human proteins using artificial neural networks that examine the sequence context of Asn-Xaa-Ser/Thr sequons.

Instructions Output format Abstract


Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Alternatively, type in Swiss-Prot ID/AC (e.g. CBG_HUMAN)

Generate graphics       Show additional thresholds (0.32, 0.75, 0.90) in the graph(s)

By default, predictions are done only on the Asn-Xaa-Ser/Thr sequons (incl. Asn-Pro-Ser/Thr)

Predict on all Asn residues - use this only if you know what you are doing!

Notes: SignalP is automatically run on all sequences. A warning is displayed if a signal peptide is not detected. In transmembrane proteins, only extracellular domains may be N-glycosylated. This is currently not checked by the NetNGlyc server. Cytoplasmic and transmembrane sequence regions may be predicted to be glycosylated - this should, of course, be ignored. One transmembrane region predictor is TMHMM.

Restrictions: At most 2,000 sequences and 200,000 amino acids per submission; each sequence not more than 4,000 amino acids (PLEASE NOTE THE NEW LIMIT!).
Confidentiality: The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Gupta R, Brunak S.
Prediction of glycosylation across the human proteome and the correlation to protein function.
Pac Symp Biocomput. 2002;:310-22.
PMID: 11928486y


Would you prefer to run NetNGlyc at your own site? NetNGlyc 1.0 is available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at


Scientific problems:        Technical problems: