Please note that this is an old version of NetOGlyc. The current version can be found at:

NetOGlyc 2.0 Server

The NetOglyc server produces neural network predictions of mucin type GalNAc O-glycosylation sites in mammalian proteins.

The current version of NetOGlyc is available at:

Instructions Output format O-GlycBase Abstract Mail server


Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Generate graphics

At most 50 sequences and 70,000 amino acids per submission; each sequence not more than 4,000 amino acids.

The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Prediction of O-glycosylation of mammalian proteins: Specificity patterns of UDP-GalNAc:-polypeptide N-acetylgalactosaminyltransferase.
J.E. Hansen, O. Lund, J. Engelbrecht, H. Bohr, J.O. Nielsen, J-E.S. Hansen, and S. Brunak.
Biochemical Journal: 308, 801-813, 1995.

O-glycbase version 2.0 - A revised database of O-glycosylated proteins.
J.E. Hansen, O. Lund, K. Rapacki and S. Brunak.
Nucleic Acids Research, 25: 278-282, 1997.

NetOglyc: Prediction of mucin type O-glycosylation sites based on sequence context and surface accessibility.
J.E. Hansen, O. Lund, N. Tolstrup, A.A. Gooley, K.L. Williams and S. Brunak.
Glycoconjugate Journal, 15: 115-130, 1998.



Would you prefer to run NetOGlyc at your own site? NetOGlyc 2.0 is available as a package on a commercial license. Currently available platforms include MIPS (under IRIX, Silicon Graphics), SPARC (under Solaris, Sun), Alpha (under OSF1) and Pentium family (under Linux). Send inquiries by e-mail to


Scientific problems:        Technical problems: