Email Prediction Server
The NetOglyc Email server produces neural network predictions of mucin type
O-glycosylation sites in mammalian proteins.
1: Press netOglyc@cbs.dtu.dk
Paste in sequences in FASTA format
(include as the first word "graphics" if you want postscript graphics back)
2: Omit anything after the last sequence (any signature will be interpreted as sequence),
or put a single '.' on a line (no other things) will make a forced end of the data, thus making it possible to
eliminate such things as signatures from the sequences. Do not send sequnces of over 2500 aa. Divide these in two!
3: Press Send Buttom
4: Results will be mailed to you.
Put you sequences in FASTA format in a file called e.g. seq.dat
On unix machines type: mail netOglyc@genome.cbs.dtu.dk < seq.dat
On PCs mail the file (saved in plain text, ASCII format) to: netOglyc@cbs.dtu.dk
6: Results will be mailed to you.
7: Inclusion of the word 'Help' in the file, make the server send you information about input and
8: Do not send more than 50 sequences at a time.
9: If the first word in the mail is "graphics" postscript graphics of results is e-mailed back to you.
For publication of results, please cite:
Prediction of O-glycosylation of mammalian proteins: Specificity patterns of
J.E. Hansen, O. Lund, J. Engelbrecht, H. Bohr, J.O. Nielsen, J-E.S. Hansen, and S. Brunak.
Biochemical Journal, 308, 801-813, 1995.
O-glycbase version 2.0 - A revised database of O-glycosylated proteins.
J.E. Hansen, O. Lund, K. Rapacki and S. Brunak.
Nucleic Acids Research, 25: 278-282, 1997.
NetOglyc: Prediction of mucin type O-glycosylation sites based on sequence context and surface accessibility
J.E. Hansen, O. Lund, N. Tolstrup, A.A. Gooley, K.L. Williams and S. Brunak.
Glycoconjugate Journal 1997, submitted.