Usage instructions

The input sequences must be in the one-letter code: 'acdefghiklmnpqrstvwyACDEFGHIKLMNPQRSTVWY'.
Note: other characters will be accepted, but not used when making the predictions.

There are two methods of input:

• Paste a single sequence or several sequences in FASTA format into the input field.
  Note: if you paste more than one sequence FASTA format must be used.

• Submit a file in FASTA format directly from your local disk. The file may contain multiple sequences.

Peptides shorter than 19 residues can produce non-reliable results. Always include 9 residues on both sides of the Ser/Thr you want evaluated. The optimal is to use the complete "native" protein sequence with the signal peptide truncated.

By default the server produces graphical output illustrating the predictions (in GIF and PostScript). The graphs can be very valuable for locating the "hot" spots in your protein. You can suppress the generation of graphics by un-checking the button labelled 'Generate graphics'.

When ready press the button labelled 'Submit'. The status of your job (either 'queued' or 'running') will be displayed and constantly updated until it terminates and the server output appears in the browser window.