The input sequences must be in the one-letter code:
Note: other characters will be accepted, but not used when making
There are two methods of input:
Both methods may be used in the same submission.
- Paste a single sequence or several sequences in
format into the input field.
Note: if you paste more than one sequence FASTA format must be used.
- Submit a file in FASTA format
directly from your local disk. The file may contain multiple sequences.
Peptides shorter than 19 residues can produce non-reliable results. Always
include 9 residues on both sides of the Ser/Thr you want evaluated.
The optimal is to use the complete "native" protein sequence with the signal
By default the server produces graphical output illustrating the predictions
(in GIF and PostScript). The graphs can be very valuable for locating the
"hot" spots in your protein. You can suppress the generation of graphics by
un-checking the button labelled 'Generate graphics'.
When ready press the button labelled 'Submit'. The status of your job
(either 'queued' or 'running') will be displayed and constantly updated until
it terminates and the server output appears in the browser window.
At any time during the wait you may enter your e-mail address and simply
leave the window. Your job will continue; you will be notified by e-mail
when it has terminated. The e-mail message will contain the URL under which
the results are stored; they will remain on the server for 24 hours for you
to collect them.