Please note that this is an old version of NetOGlyc. The current version can be found at:

NetOGlyc 3.0 Server

The NetOglyc server produces neural network predictions of mucin type GalNAc O-glycosylation sites in mammalian proteins.
This version replaces NetOGlyc 2.0 (click here for that version).

View the version history of this server.

Instructions Output format Article abstract


Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Generate graphics       Run signal peptide check

Notes: SignalP is automatically run on all sequences. A warning is displayed if a signal peptide is not detected. In transmembrane proteins, only extracellular domains may be O-glycosylated with mucin-type GalNAc. This is currently not checked by the NetOGlyc server. Cytoplasmic and transmembrane sequence regions may be predicted to be glycosylated - this should, of course, be ignored. One transmembrane region predictor is TMHMM.

Restrictions: At most 50 sequences and 200,000 amino acids per submission; each sequence not more than 4,000 amino acids.

Confidentiality: The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Prediction, conservation analysis and structural characterization of mammalian mucin-type O-glycosylation sites
K. Julenius, A. Mølgaard, R. Gupta and S. Brunak.
Submitted to Glycobiology

View the abstract.


Would you prefer to run NetOGlyc at your own site? The software package is in preparation. Send inquiries by e-mail to


Scientific problems:        Technical problems: