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NetOGlyc 3.1 Server

The NetOglyc server produces neural network predictions of mucin type GalNAc O-glycosylation sites in mammalian proteins.
This version replaces NetOGlyc 2.0 (click here for that version).

View the version history of this server.

Instructions Output format Article abstract


Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Generate graphics       Run signal peptide check

Notes: SignalP is automatically run on all sequences. A warning is displayed if a signal peptide is not detected. In transmembrane proteins, only extracellular domains may be O-glycosylated with mucin-type GalNAc. This is currently not checked by the NetOGlyc server. Cytoplasmic and transmembrane sequence regions may be predicted to be glycosylated - this should, of course, be ignored. One transmembrane region predictor is TMHMM.

Restrictions: At most 50 sequences and 200,000 amino acids per submission; each sequence not more than 4,000 amino acids.

Confidentiality: The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Prediction, conservation analysis and structural characterization of mammalian mucin-type O-glycosylation sites
K. Julenius, A. Mølgaard, R. Gupta and S. Brunak.
Glycobiology, 15:153-164, 2005.

View the abstract or the full text.


Would you prefer to run NetOGlyc at your own site? NetOGlyc 3.1 is available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at


Scientific problems:        Technical problems: