Services are gradually being migrated to
Please try out the new site.

Please note that this is an old version of NetPhos. The current version can be found at:

NetPhos 2.0 Server

The NetPhos 2.0 server produces neural network predictions for serine, threonine and tyrosine phosphorylation sites in eukaryotic proteins.

Kinase specific phosphorylation predictions are available at:

Instructions Output format PhosphoBase Abstract


Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Predict on: tyrosine serine threonine

Generate graphics

At most 2000 sequences and 200,000 amino acids per submission; each sequence not less than 15 and not more than 4,000 amino acids.

The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Sequence- and structure-based prediction of eukaryotic protein phosphorylation sites.
Blom, N., Gammeltoft, S., and Brunak, S.
Journal of Molecular Biology: 294(5): 1351-1362, 1999.

View the abstract.


Would you prefer to run NetPhos at your own site? NetPhos 3.1 is available as a stand-alone software package, combining the functionality of the service above (v. 2.0, generic predictions) and NetPhosK (kinase specific predictions). Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at

Please note that NetPhos 3.1 is not yet available on the WWW; however, it produces the same predictions as NetPhos 2.0 and NetPhosK.


Scientific problems:        Technical problems: