Services are gradually being migrated to
In the near future, will be retired. Please try out the new site.

Please note that this is an old version of NetPhos. The current version can be found at:

NetPhos 2.0 Server

The NetPhos 2.0 server produces neural network predictions for serine, threonine and tyrosine phosphorylation sites in eukaryotic proteins.

Kinase specific phosphorylation predictions are available at:

Instructions Output format PhosphoBase Abstract


Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Predict on: tyrosine serine threonine

Generate graphics

At most 2000 sequences and 200,000 amino acids per submission; each sequence not less than 15 and not more than 4,000 amino acids.

The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Sequence- and structure-based prediction of eukaryotic protein phosphorylation sites.
Blom, N., Gammeltoft, S., and Brunak, S.
Journal of Molecular Biology: 294(5): 1351-1362, 1999.

View the abstract.


Would you prefer to run NetPhos at your own site? NetPhos 3.1 is available as a stand-alone software package, combining the functionality of the service above (v. 2.0, generic predictions) and NetPhosK (kinase specific predictions). Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at

Please note that NetPhos 3.1 is not yet available on the WWW; however, it produces the same predictions as NetPhos 2.0 and NetPhosK.


Scientific problems:        Technical problems: