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Usage instructions


The input sequences must be in the one-letter code: 'acdefghiklmnpqrstvwyACDEFGHIKLMNPQRSTVWY'.
Note: other characters will be accepted, but not used when making the predictions.

There are two methods of input:

  • Paste a single sequence or several sequences in FASTA format into the input field.
    Note: if you paste more than one sequence FASTA format must be used.

  • Submit a file in FASTA format directly from your local disk. The file may contain multiple sequences.
Both methods may be used in the same submission.

Peptides shorter than 9 residues can produce non-reliable results. Always include 4 residues on both sides of the Tyr/Ser/Thr you want evaluated. The optimal is to use the complete "native" protein sequence.

By default the server predicts phosphorylation sites for all the three amino acids serine, threonine and tyrosine. You can limit the scope of prediction by un-checking the buttons of your choice.

By default the server produces graphical output illustrating the predictions. You can suppress that by un-checking the button labelled 'Generate graphics'.

When ready press the button labelled 'Submit'. The status of your job (either 'queued' or 'running') will be displayed and constantly updated until it terminates and the server output appears in the browser window.

At any time during the wait you may enter your e-mail address and simply leave the window. Your job will continue; you will be notified by e-mail when it has terminated. The e-mail message will contain the URL under which the results are stored; they will remain on the server for 24 hours for you to collect them.


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