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NetPhos 3.1 Server

The NetPhos 3.1 server predicts serine, threonine or tyrosine phosphorylation sites in eukaryotic proteins using ensembles of neural networks. Both generic and kinase specific predictions are performed. The generic predictions are identical to the predictions performed by NetPhos 2.0. The kinase specific predictions are identical to the predictions by NetPhosK 1.0. Predictions are made for the following 17 kinases:

ATM, CKI, CKII, CaM-II, DNAPK, EGFR, GSK3, INSR, PKA, PKB, PKC, PKG, RSK, SRC, cdc2, cdk5 and p38MAPK.


See the version history of this server.


NOTE: the online service at http://www.cbs.dtu.dk/services/NetPhosK is currently off-line;
for the kinase specific predictions this service should be used instead.


Instructions Output format PhosphoBase

SUBMISSION

Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Residues to predict serine threonine tyrosine all three

For each residue display only the best prediction
Display only the scores higher than

Output format classical GFF
Generate graphics

Restrictions:
At most 2000 sequences and 200,000 amino acids per submission; each sequence not less than 15 and not more than 4,000 amino acids.

Confidentiality:
The sequences are kept confidential and will be deleted after processing.


CITATIONS

For publication of results, please cite:

Generic predictions:

Sequence- and structure-based prediction of eukaryotic protein phosphorylation sites.
Blom, N., Gammeltoft, S., and Brunak, S.
Journal of Molecular Biology: 294(5): 1351-1362, 1999.

PMID: 10600390

Kinase specific predictions:

Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence.
Blom N, Sicheritz-Ponten T, Gupta R, Gammeltoft S, Brunak S.
Proteomics: Jun;4(6):1633-49, review 2004.

PMID: 15174133


PORTABLE VERSION

Would you prefer to run NetPhos at your own site? NetPhos 3.1 is available as a stand-alone software package. Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at software@cbs.dtu.dk.


GETTING HELP

Scientific problems:        Technical problems: