NetSurfP ver. 1.1 - Protein Surface Accessibility and Secondary Structure Predictions

Version 1.1: Caching system has been implemented.

View the version history of this server.

NetSurfP server predicts the surface accessibility and secondary structure of amino acids in an amino acid sequence.
The method also simultaneously predicts the reliability for each prediction, in the form of a Z-score. The Z-score is related to the surface prediction, and not the secondary structure.

Instructions Output format Article abstract

Speed: Approx 5 min per sequence, but much faster if sequence has been cached previously.

Paste in sequence data (maximum 1500 sequences)

or upload sequence data

Valid format examples: Fasta

All sequences must be submitted in amino acid format and have a unique sequence id!
Do not cache sequence profile and prediction results.
Instructions: Paste in or upload the input FASTA file and make sure that all sequences have a unique sequence id.

Please read the CBS access policies for information about limitations on the daily number of submissions.


For publication of results, please cite:

A generic method for assignment of reliability scores applied to solvent accessibility predictions.
Bent Petersen, Thomas Nordahl Petersen, Pernille Andersen, Morten Nielsen and Claus Lundegaard1.
BMC Structural Biology 2009, 9:51 doi:10.1186/1472-6807-9-51.

View the abstract: abstract
The electronic version of this article is found here: download


Would you prefer to run NetSurfP at your own site?. NetSurfP 1.0 is available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for IRIX and Linux. There is a download page for academic users; other users are requested to contact Bent Petersen at


Scientific problems:        Technical problems: