OligoWiz 1.0 Server
This server performs intelligent design of oligonucleotides for DNA
OligoWiz is implemented as a client-server solution, which consist of an
easy to use Graphical User Interface (the client - written in Java) which
delegates the computationally intensive operations to a server (which by
default is hosted at CBS on a powerfull Multi-CPU SGI UNIX server).
All the user needs to do is to download and start
The client is written in Java 1.3.1 with portability in mind. It is tested
on MacOS X, Linux and Windows, but should work on any platform with Java
1.3.1 or better. The client communicates with server using the HTTP protocol.
Example screenshot form the client program
New version: v.1.0.8 (9th of April , 2003) - view changes.
The most recent version of the OligoWiz client can be downloaded
here as a Java Archive (JAR).
Note: for some browsers you may have to right click and choose
"Save Target As..."
Also, some browsers have been reported to rename the OligoWiz.jar file to *.zip.
If this happens the file must be re-rename to *.jar.
Browse all releases.
If Java is allready installed, starting the OligoWiz client is as easy
as double-clicking the file (Windows
and MacOS X
), or typing
java -jar OligoWiz.jar
the command prompt (Unix
Out of Memory!
If you run large sets of sequences on OligoWiz you may experience memory problems.
In most cases this can be fixed by executing OligoWiz in the following way (in a prompt):
java -jar -Xmx300m OligoWiz.jar
Where "300m" is the maximum amount of memory allocated by OligoWiz.jar (300 megabytes)
In order to set up OligoWiz to access our server through a proxy, the proxy parameters
need to be parsed to the JVM on the command prompt:
java -jar -Dhttp.proxyHost=proxyhost -Dhttp.proxyPort=portNumber
OligoWiz.jar (Put in you relevant proxyhost/port number).
A suitable version of Java (choose J2SE version 1.3.1 or newer) can be
The OligoWiz client can be used free of charge for both academic and commercial
use, in combination with the OligoWiz server hosted at CBS. However, please
see the access policies
of the CBS server.
SAMPLE DATA SET
The sample input data set is
a FASTA file containing 30 Bacillus subtilis coding regions. It can
be used as a test input for the OligoWiz client program.
For publication of results, please cite:
Design of oligonucleotides for microarrays and perspectives for design
of multi-transcriptome arrays.
Henrik Bjørn Nielsen, Rasmus Wernersson and Steen Knudsen.
Nucleic Acids Research, 2003, Vol. 31, No. 13 3491-3496
Would you prefer to run the OligoWiz server at your own site? OligoWiz 1.0
server is available as a package on a commercial license.
Send inquiries by e-mail to