OligoWiz 2.0 is a powerful tool for microarray probe design that allows
for integration of sequence annotation such as exon/intron structure,
UTR regions, TSS, etc. In addition to probe selection according to a
series of probe quality parameters, cross-hybridization, Δ Tm, position
in transcript, probe folding and low-complexity, the program
facilitates automatic placement of probes relative to the sequence
annotation. The program also supports automatic placement of multiple
probes per transcript. Together these facilities make advanced probe
design feasible for scientists inexperienced in computerizes
information management. Furthermore, we show that probes designed using
OligoWiz 2.0 give rise to consistent hybridization results.
OligoWiz 2.0 - integrating sequence feature annotation into design of microarray probes
Rasmus Wernersson and Henrik Bjørn Nielsen.
Nucleic Acids Research, 2005, Vol. 33, Web Server issue W611-W615
Design of oligonucleotides for microarrays and perspectives for design
of multi-transcriptome arrays.
Henrik Bjørn Nielsen, Rasmus Wernersson and Steen Knudsen.
Nucleic Acids Research, 2003, Vol. 31, No. 13 3491-3496
Rasmus Wernersson, personal webpage:
Henrik Bjørn Nielsen and Rasmus Wernersson,