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Article abstract


ABSTRACT

OligoWiz 2.0 is a powerful tool for microarray probe design that allows for integration of sequence annotation such as exon/intron structure, UTR regions, TSS, etc. In addition to probe selection according to a series of probe quality parameters, cross-hybridization, Δ Tm, position in transcript, probe folding and low-complexity, the program facilitates automatic placement of probes relative to the sequence annotation. The program also supports automatic placement of multiple probes per transcript. Together these facilities make advanced probe design feasible for scientists inexperienced in computerizes information management. Furthermore, we show that probes designed using OligoWiz 2.0 give rise to consistent hybridization results.

REFERENCE

OligoWiz 2.0 - integrating sequence feature annotation into design of microarray probes
Rasmus Wernersson and Henrik Bjørn Nielsen.
Nucleic Acids Research, 2005, Vol. 33, Web Server issue W611-W615

Design of oligonucleotides for microarrays and perspectives for design of multi-transcriptome arrays.
Henrik Bjørn Nielsen, Rasmus Wernersson and Steen Knudsen.
Nucleic Acids Research, 2003, Vol. 31, No. 13 3491-3496

Rasmus Wernersson, personal webpage: http://www.cbs.dtu.dk/~raz/


CORRESPONDENCE

Henrik Bjørn Nielsen and Rasmus Wernersson,