PHUSER version 2

Brief instructions:
Select a complete predefined fusion cassette on the list below - this will provide all the information needed. If you don't need to clone your fused construct into a vector, you can select LINEAR or CIRCULAR, and no cassette will be used

Here you can select a predefined fusion cassette (see details below)

1: Name: PacI/Nt.BbvCI Forward strand: GCTGAGGGTTTAATTAAGACCTCAGC Reverse strand: CGACTCCCAAATTAATTCTGGAGTCG 2: Name: XbaI2/Nt.BbvCI Forward strand: GCTGAGGGAAAGTCTAGAGGATCCTCTAGATGTCTCCTCAGC Reverse strand: CGACTCCCTTTCAGATCTCCTAGGAGATCTACAGAGGAGTCG 3: Name: PmeI/Nb.BbvCI Forward strand: CCTCAGCCGTTTAAACAGCTGAGG Reverse strand: GGAGTCGGCAAATTTGTCGACTCC 4: Name: AsiSI/Nb.BSMI Forward strand: GAATGCGTGCGATCGCGTGCATTC Reverse strand: CTTACGCACGCTAGCGCACGTAAG 5: Name: AsiSI/Nb.BtsI Forward strand: GCAGTGAGAGCGATCGCAGACACTGC Reverse strand: CGTCACTCTCGCTAGCGTCTGTGACG

PHUSER version 2 - beta

STEP 1: Input sequences

Paste in one or more DNA sequences in FASTA format

or upload DNA sequences in FASTA format

STEP 2: Output construct

STEP 3: PCR experimental conditions

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