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Promoter 2.0 Prediction Server

Promoter2.0 predicts transcription start sites of vertebrate PolII promoters in DNA sequences. It has been developed as an evolution of simulated transcription factors that interact with sequences in promoter regions. It builds on principles that are common to neural networks and genetic algorithms.

Instructions Output format Abstract Performance


Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Full output

Restrictions: At most 50 sequences and 1,500,000 nucleotides in toto per submission.

Confidentiality: The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Promoter 2.0: for the recognition of PolII promoter sequences.
Steen Knudsen
Bioinformatics 15, 356-361, 1999.


The software is copyright © 1998 by Steen Knudsen and all rights are reserved. Promoter 2.0 is also available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at


Scientific problems:        Technical problems: