ProtFun 2.2 Server

Prediction of cellular role, enzyme class and Gene Ontology category

The ProtFun 2.2 server produces ab initio predictions of protein function from sequence. The method queries a large number of other feature prediction servers to obtain information on various post-translational and localizational aspects of the protein, which are integrated into final predictions of the cellular role, enzyme class (if any), and selected Gene Ontology categories of the submitted sequence.

It is possible to inspect the individual feature predictions used and integrated by ProtFun.

View the version history of this server.

Instructions Output format Abstract


Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Show the individual features (PTM:s, sorting signals etc.) in the output
Show the prediction process as it progresses

Restrictions:       *** NEW: 50 sequences per submission ***
At most 50 sequences and 75,000 amino acids per submission; each sequence not less than 21 and not more than 4,000 amino acids.

The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Ab initio prediction of human orphan protein function from post-translational modifications and localization features.
L. Juhl Jensen, R. Gupta, N. Blom, D. Devos, J. Tamames, C. Kesmir, H. Nielsen, H. H. Stærfeldt,
K. Rapacki, C. Workman, C. A. F. Andersen, S. Knudsen, A. Krogh, A. Valencia and S. Brunak.

J. Mol. Biol., 319:1257-1265, 2002.

View the abstract.

Prediction of human protein function according to Gene Ontology categories.
Lars Juhl Jensen, Hans-Henrik Stærfeldt and Søren Brunak.
Bioinformatics, 19:635-642, 2003.

View the abstract.


Scientific problems:        Technical problems: