RevTrans 1.4 Server

Paste in DNA sequences

Upload file containing DNA sequences

Optional: Paste in peptide alignment

Optional: Upload peptide alignment

Instructions: Paste in or upload DNA sequences and hit "Submit query". The RevTrans server will then virtually translate the sequences and align the resulting peptide sequences using Dialign2 with default settings. Finally RevTrans constructs a multiple DNA alignment using the peptide alignment as a scaffold. The translation process is by default done using the Standard Genetic Code (alternative translation tables can be selected) and has support for the full IUPAC alphabet of degenerate nucleotides.

If you want more control over the alignment process RevTrans also accepts user provided peptide alignments. This will give you the opportunity to use your preferred alignment software and to optimize the parameters. If you need to translate your DNA sequences prior to alignment this can be done by using the "Translate only" button above.

When providing your own peptide alignment, RevTrans will accept arbitrarily large input files.

Please read the CBS access policies for information about limitations on the daily number of submissions.

Data format, DNA sequences

Data format, aligned peptide sequences

Output format

Gap-In

Match DNA and peptide sequences by:

Gap-Out

Translation table:

The translation tables are adapted from the NCBI taxonomy web site.

For a detailed description of each genetic code, please consult the following web page at NCBI: The Genetic Codes . [Main site: Taxonomy]

Alignment method

Verbose