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SecretomeP 1.0f Server

Prediction of non-classical protein secretion

The SecretomeP 1.0f server produces ab initio predictions of non-classical i.e. not signal peptide triggered protein secretion in eukaryotes. The method queries a large number of other feature prediction servers to obtain information on various post-translational and localizational aspects of the protein, which are integrated into the final secretion prediction.

View the version history of this server.

Instructions Output format Article abstract Data sets


Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Sort the output by prediction score    Report the progress of the prediction process

At most 500 sequences and 200,000 amino acids per submission; each sequence not less than 15 and not more than 4,000 amino acids.

The sequences are kept confidential and will be deleted after processing.


For publication of results, please cite:

Feature based prediction of non-classical and leaderless protein secretion.
J. Dyrløv Bendtsen, L. Juhl Jensen, N. Blom, G. von Heijne and S. Brunak.
Protein Eng. Des. Sel., 17(4):349-356, 2004

View the abstract.


Would you prefer to run SecretomeP at your own site? SecretomeP 1.0 is available as a package on a commercial license. Currently available platforms include MIPS (under IRIX, Silicon Graphics) and Pentium family (under Linux and CYGWIN). Send inquiries by e-mail to


Scientific problems:        Technical problems: