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SignalP V1.1
World Wide Web Prediction Server
Center for Biological Sequence Analysis
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The SignalP mail server
predicts the presence and location of signal peptide cleavage sites in
amino acid sequences from different organisms: Gram-positive
prokaryotes, Gram-negative prokaryotes, and eukaryotes. The method
incorporates a prediction of cleavage sites and a signal
peptide/non-signal peptide prediction based on a combination of several
artificial neural networks.
If your browser supports forms, it is faster
and more convenient to use the SignalP World Wide Web server
for prediction of single sequences.
The mail server takes longer time to return predictions than the WWW
server, but it is possible to submit several sequences in one mail.
Submit
INSTRUCTIONS
- Enter one or more sequences in the mail window.
The sequences should be in `FASTA' format:
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Each sequence must be preceded by a sequence header line starting by
the symbol `>'
immediately followed by a name (identifier) of the sequence. The rest
of the header line is ignored.
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The sequences must be written using the one letter
abbreviations for the amino acids:
`acdefghiklmnpqrstvwy' or
`ACDEFGHIKLMNPQRSTVWY'.
Other letters will be converted to `X' and treated as unknown
amino acids.
Other characters, such as blanks and
numbers, will simply be ignored.
N.B.:
It is recommended that the N-terminal part only (not more than 50-70
amino acids) of the sequences is submitted. A longer sequence will
increase the risk of false positives and make the graphical output
difficult to read.
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Before the first sequence, the following keywords may appear:
- `euk', `gram-', or `gram+'
- Use networks trained on sequences from eukaryotes, Gram-negative
prokaryotes, or Gram-positive prokaryotes, respectively. If none of
these keywords are found, predictions from all three network ensembles
will be returned.
- `postscript' or `graphics'
- Request a graphical output (in Postscript format) of the prediction.
The Postscript file will be sent as a separate message. Before viewing
the postscript output, the mail headers must be removed.
- `help'
- Send the help file.
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A line consisting of only a `.' (dot) terminates the sequences,
and the rest of the message is discarded. This is useful if your mailer
automatically appends a signature.
- Mail the sequences to signalp@cbs.dtu.dk
N.B. Be sure that your World Wide Web browser's mail system is
set up correctly. Your e-mail adress must be correct in order to
receive prediction output from the mail server.
- You will receive a mail containing the prediction, or possibly
error messages, from the server. Response time depends on system
load.
EXAMPLE
If you want a prediction of signal peptides in two human protein
sequences, and a graphical representation of the results, your
mail might look like this:
euk
graphics
>10KS_HUMAN
MKLAVTLTLV TLALCCSSAS AEICPSFQRV IETLLMDTPS SYEAAMELFS
>1B05_HUMAN
MRVTAPRTLL LLLWGAVALT ETWAGSHSMR YFYTAMSRPG RGEPRFITVG
.
COMMENTS AND SUGGESTIONS
Mail to:
PROBLEMS
Mail to:
Last change: December 2, 1996,
Kristoffer Rapacki
