SignalP 3.0 Server

SignalP 3.0 server predicts the presence and location of signal peptide cleavage sites in amino acid sequences from different organisms: Gram-positive prokaryotes, Gram-negative prokaryotes, and eukaryotes. The method incorporates a prediction of cleavage sites and a signal peptide/non-signal peptide prediction based on a combination of several artificial neural networks and hidden Markov models.

View the version history of this server. All the previous versions are available on line, for comparison and reference.

NOTE: This is not the newest version of SignalP. To use the current version, please go to http://www.cbs.dtu.dk/services/SignalP/

Background

Article abstracts

Instructions

Output format

CITATIONS

For publication of results, please cite:

• Current version:

  Improved prediction of signal peptides: SignalP 3.0.
  Jannick Dyrløv Bendtsen, Henrik Nielsen, Gunnar von Heijne and Søren Brunak.
  J. Mol. Biol., 340:783-795, 2004.

  View the abstract.         Download the full article in PDF.

• Original paper:

  Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
  Henrik Nielsen, Jacob Engelbrecht, Søren Brunak and Gunnar von Heijne.
  Protein Engineering, 10:1-6, 1997.

  View the abstract.

• If you specifically use the SignalP-HMM output, please also cite:

  Prediction of signal peptides and signal anchors by a hidden Markov model.
  Henrik Nielsen and Anders Krogh.
  Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology (ISMB 6),
  AAAI Press, Menlo Park, California, pp. 122-130, 1998.

  View the abstract.

• A paper about using SignalP and other protein subcellular localization prediction methods:
  
  Locating proteins in the cell using TargetP, SignalP, and related tools
  Olof Emanuelsson, Søren Brunak, Gunnar von Heijne, Henrik Nielsen
  Nature Protocols 2, 953-971 (2007).
  
  is available for download - please click here to access the paper and supplementary materials.

PORTABLE VERSION

Would you prefer to run SignalP at your own site? SignalP 3.0 is available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users where you can get the newest SignalP version; if you specifically want the 3.0 version, please send a mail to Support. Non-academic users are requested to contact CBS Software Package Manager at software@cbs.dtu.dk.

GETTING HELP