SignalP 4.1 Server

SignalP 4.1 server predicts the presence and location of signal peptide cleavage sites in amino acid sequences from different organisms: Gram-positive prokaryotes, Gram-negative prokaryotes, and eukaryotes. The method incorporates a prediction of cleavage sites and a signal peptide/non-signal peptide prediction based on a combination of several artificial neural networks.

View the version history of this server. All the previous versions are available on line, for comparison and reference.

NEW: The portable version of SignalP 4.1, previously only available for Mac (Darwin), Linux, and IRIX, is now also available for Windows systems. Academic users: select the "CYGWIN" option at the download page. Cygwin or MobaXterm is required to install SignalP under Windows. For details, read the installation instructions.

FAQ

Article abstracts

Instructions

Output format

Performance

Data

SignalP 4.0: discriminating signal peptides from transmembrane regions
Thomas Nordahl Petersen, Søren Brunak, Gunnar von Heijne & Henrik Nielsen
Nature Methods, 8:785-786, 2011

doi: 10.1038/nmeth.1701
PMID: 21959131
Supplementary materials: nmeth.1701-S1.pdf

Other relevant papers:

• Original paper (version 1.0):

  Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
  Henrik Nielsen, Jacob Engelbrecht, Søren Brunak and Gunnar von Heijne.
  Protein Engineering, 10:1-6, 1997.

  View the abstract.

• SignalP-HMM (version 2.0):

  Prediction of signal peptides and signal anchors by a hidden Markov model.
  Henrik Nielsen and Anders Krogh.
  Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology (ISMB 6),
  AAAI Press, Menlo Park, California, pp. 122-130, 1998.

  View the abstract.

• Version 3.0:

  Improved prediction of signal peptides: SignalP 3.0.
  Jannick Dyrløv Bendtsen, Henrik Nielsen, Gunnar von Heijne and Søren Brunak.
  J. Mol. Biol., 340:783-795, 2004.

  View the abstract.         Download the full article in PDF.

• Paper about using SignalP and other protein subcellular localization prediction methods:

  Locating proteins in the cell using TargetP, SignalP, and related tools
  Olof Emanuelsson, Søren Brunak, Gunnar von Heijne, Henrik Nielsen
  Nature Protocols 2:953-971 (2007).
  

  Access the paper and supplementary materials.

PORTABLE VERSION

Would you prefer to run SignalP at your own site? SignalP 4.1 is available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for Mac OS X (Darwin), Windows (Cygwin), and the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at software@cbs.dtu.dk.

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