SigniSite 1.1 Server

SigniSite 1.1 identifies amino acid residues, which in a FASTA multiple alignment are found to be significantly associated with the phenotype of the data set. The phenotype is represented by a continuous numerical parameter placed last in the identifier of each sequence. For test of server functionality, please see 'Sample data'.

Instructions Output format Article abstract


Sample data

Please note that non-amino acid residue characters, will not be included in the evaluation,
i.e. anything other than 'ARNDCQEGHILKMFPSTWYV' will be excluded.
Sample data for test of server functionality is available. The sample data will reproduce the results used on 'output format' page.
To load the sample data, simply click the 'Load sample data' button below and 'submit':
Paste a multiple alignment in FASTA format into the field below:
Submit a multiple alignment file in FASTA format directly from your local disk:


Instructions on the meaning of the parameters below, can be found here
Significance threshold α =
Method for correction for multiple testing
Choose sorting of numerical values
Unique sequence ID for relative numbering
Type of logo
Should gaps be ignored? By default SigniSite-1.1 consideres gaps as amino acid residue #21


The sequences are kept confidential and will be deleted after analysis.


Scientific problems:        Technical problems and suggestions: