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TargetP Server v1.01

Prediction of subcellular location

TargetP v1.01 predicts:

1. Subcellular location
TargetP predicts the subcellular location of eukaryotic protein sequences. The subcellular location assignment is based on the predicted presence of any of the N-terminal presequences chloroplast transit peptide (cTP), mitochondrial targeting peptide (mTP) or secretory pathway signal peptide (SP).

2. Cleavage site
If opted for, TargetP provides a potential cleavage site for sequences predicted to contain a cTP, mTP or SP.

NEW: Version 1.01 of TargetP performs the cleavage site prediction also for SP:s (which version 1.0 did not do). TargetP uses SignalP for this purpose. No other changes have been implemented.

Here you can see predictions of A. thaliana and H. sapiens sets

Instructions Output format Data Sets Abstract

SUBMISSION

Submission of one single sequence, or local file containing one or several sequence(s) in fasta format.

The input limits are as follows:

  • At most 2000 sequences and 200,000 amino acids per submission; each sequence not more than 4,000 amino acids.

All of these limits have to be satisfied in each run.

Single sequence:

Sequence name (optional):


Sequence (one letter code):


Local file:

File name (fasta format):

TargetP can handle up to 1500 sequences in one file (max 2x106 residues in total).


Customize the run:

1. Origin of sequences:
Non plant
Plant

2. Cleavage sites:
Perform cleavage site predictions.

3. Choose cutoffs:
No cutoffs; winner-takes-all (default).
Specificity >0.95. (A predefined set of cutoffs that yielded this specificity on the TargetP test sets).
Specificity >0.90. (A predefined set of cutoffs that yielded this specificity on the TargetP test sets).
Define your own cutoffs (0.00 - 1.00): cTP: mTP: SP: other:



CITATIONS

Reference: If you are using the prediction of localization, or if you are using the prediction of cleavage sites ("TPlen") of predicted cTP:s or mTP:s ("C" and "M", respectively, on results page), please cite:

Olof Emanuelsson, Henrik Nielsen, Søren Brunak, and Gunnar von Heijne: "Predicting subcellular localization of proteins based on their N-terminal amino acid sequence", J. Mol. Biol. 300, 1005-1016 (2000).
Abstract - JMB Online

Note: If you are using the results of the prediction of cleavage sites ("TPlen") of predicted SP:s ("S" on results page), please also cite:
Henrik Nielsen, Jacob Engelbrecht, Søren Brunak, and Gunnar von Heijne: "Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites", Prot. Eng. 10, 1-6 (1997).




GETTING HELP

Scientific problems:        Technical problems: