Events News Research CBS CBS Publications Bioinformatics
Staff Contact About Internal CBS CBS Other

CBS >> CBS Prediction Servers >> TargetP

TargetP 1.1 Server

TargetP 1.1 predicts the subcellular location of eukaryotic proteins. The location assignment is based on the predicted presence of any of the N-terminal presequences: chloroplast transit peptide (cTP), mitochondrial targeting peptide (mTP) or secretory pathway signal peptide (SP).

For the sequences predicted to contain an N-terminal presequence a potential cleavage site can also be predicted.

NOTE 1:   TargetP uses ChloroP and SignalP to predict cleavage sites for cTP and SP, respectively.

NOTE 2:   The method has been tested on A. thaliana and H. sapiens sets; see the results.

New: the paper about using TargetP and other protein subcellular localization prediction methods:

Locating proteins in the cell using TargetP, SignalP, and related tools
Olof Emanuelsson, Søren Brunak, Gunnar von Heijne, Henrik Nielsen
Nature Protocols 2, 953-971 (2007).

is now again available for download - please click here.


Instructions Output format Article abstract Data sets

SUBMISSION

Paste a single sequence or several sequences in FASTA format into the field below:

Submit a file in FASTA format directly from your local disk:

Organism group
Non-plant
Plant 		Prediction scope
Perform cleavage site predictions

Cutoffs
no cutoffs; winner-takes-all (default)
specificity >0.95 (predefined set of cutoffs that yielded this specificity on the TargetP test sets)
specificity >0.90 (predefined set of cutoffs that yielded this specificity on the TargetP test sets)
define your own cutoffs (0.00 - 1.00):   cTP:   mTP:   SP:   other:

Restrictions:
At most 2,000 sequences and 200,000 amino acids per submission; each sequence not more than 4,000 amino acids.

Confidentiality:
The sequences are kept confidential and will be deleted after processing.

CITATIONS

For publication of results, please cite:

Predicting subcellular localization of proteins based on their N-terminal amino acid sequence.
Olof Emanuelsson, Henrik Nielsen, Søren Brunak and Gunnar von Heijne.
J. Mol. Biol., 300: 1005-1016, 2000.

View the abstract.

If you have used the predictions of the signal peptide cleavage sites please also cite:

Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.
Henrik Nielsen, Jacob Engelbrecht, Søren Brunak and Gunnar von Heijne.
Protein Engineering, 10:1-6, 1997.

View the abstract.

PORTABLE VERSION

Would you prefer to run TargetP at your own site? TargetP 1.1 is available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at software@cbs.dtu.dk.

GETTING HELP

Scientific problems:        Technical problems:       

This file was last modified Thursday 24th of February 2011 16:05:59 GMT