deFUME 1.0 - Dynamic Exploration of Functional Metagenomics Sequencing Data

deFUME takes ab1 files as input, in order to upload these to the server you first need to compress them in a zip or tar.gz file yourself. Read here how to do this.

Raw sanger sequencing reads (ab1. format) compressed as zip or tar.gz.
       

Pre-assembled sequencing data

Load example data set.

PRIMER SEQUENCES

In order for deFUME to know which ab1 file is a forward and which is a reverse read you can enter a "primer pattern" below. For example if all your forward reads contain _F_ in the filename, enter that here. Separate multiple entries by comma, e.g. _OGEN41_,_F_

Forward primer pattern: Optional

Reverse primer pattern: Optional

E-MAIL ADDRESS

deFUME uses the Interpro database at EMBL-EBI and this requires a valid mail address. If you don't provide an valid e-mail address deFUME will not include any GO annotations and Interpro links, severly limiting the vizualization of your dataset

Email for InterPro queries: Optional

CLONING VECTOR SEQUENCE(S)

or upload a file in FASTA format directly from your local disk:






Base calling error rate:
Accuracy of base calls expressed as error probability.
The standard probability is 0.01, which corresponds to
a base call probability of 99% (or 1 error in 100 bases).
Optional