Please use our new tool: AMUSER
-integrating all functionality of PHUSER and a lot more.
|PHUSER 1.2 offers quick and easy design of PCR optimized primers for USER fusion of custom DNA fragments.|
|Instructions||Output format||Software download||Article abstract|
Please read the CBS access policies for information about limitations on the daily number of submissions. The command-line version of PHUSER is free software, and be downloaded here: Software Download.
The sequences are kept confidential and will be deleted after processing.
For publication of results, please cite:
Lars Rønn Olsen, Niels Bjørn Hansen, Mads Tvillinggaard Bonde, Hans Jasper Genee, Dorte Koefoed Holm, Simon Carlsen, Bjarne Gram Hansen, Kiran Raosaheb Patil, Uffe Mortensen, Rasmus Wernersson
PHUSER (Primer Help for USER): A novel tool for USER fusion primer design
Nucl. Acids Res. 2011, accepted for publication
View the abstract.
Keywords: Polymerase Chain Reaction, PCR, Cloning, Primers, Uracil Specific Exision Reagent (USER) fusion, USER fusion, gene fusion, primer design.
The commandline version of PHUSER is open source software (GPL license) and can be downloaded here.
If you require PHUSER a commerical license, please contact