This lecture is about DNA structural analysis of the Escherichia coli genome. In a real sense, it is a continuation of Tuesday's talk. In the first part, I will discuss the DNA Structural Atlas, and talk about the organisation of the E. coli chromosome. Then, in the second half, I will go into more detail about gene expression analysis within E. coli.
The DNA Structural AtlasThe Genome Atlas (combined view)
The Base-Composition Atlas
The DNA Repeat Atlas
Escherichia coli is probably the best characterised organism.
There are 4085 predicted genes in Escherichia coli strain K-12 isolate W3110.
There are 4289 predicted genes in Escherichia coli strain K-12 isolate MG1665.
There are about 5100 predicted genes in Escherichia coli strain O157:H7 isolate EDL933 (enterohemorrhagic pathogen).
Roughly 2600 genes have been found to be expressed in Escherichia coli strain K-12 cells, under standard laboratory growth conditions.
About 2100 spots can be seen on 2-D protein gels.
Very roughly 1000 different genes (only about 600 mRNA transcripts) are expressed at "detectable levels" in E. coli cells grown in LB media.
Only about 350 proteins exist at concentrations of > 100 copies per cell. (These make up 90% of the total protein in E.coli!)
Link to the "DNA Structural Atlas" electronic poster. (For the 3rd Annual Conference on Computational Genomics, held at the Renaissance Harborplace Hotel in Baltimore, Maryland, USA (18 - 21 November, 1999).