Consider the following "typical" (?) family:
 

 

You work for an insurance company, and have been given the following sequence, isolated the DNA from the youngest child.  What does this mean, in terms of the health prospects of this child?
 
 

        1 tcgactcgat ctttcatggg tcaaataaac acagaattga tagaatattt taataatcta
       61 acaaagatga tttaataaat atttattaaa ttttgttctc tagaaataag caatacactt
      121 ttttaaggag ccatgaagca tttagaaaaa aattgatcat atattatatc tcaaagaaac
      181 catattaaat tatgaaatat aaagtgttaa cagcctccat gttctgccta cactgaaata
      241 gagctagaca ttaatagcaa aagtttaaat gcaaaaatca gtagcttgga aaagagaaat
      301 aatgataaca aaaaccaaat ttttttctaa aaactccaga ttcaaacagg aaaggaaact
      361 atataataaa ctgttaggag agtaagaact ctatgtatca atattttaaa tggctaaagc
      421 tgtatttagc agaaaattaa tagccctgag aactttcatt attaaaggag agtaacaaaa
      481 tcaatctgtg gaaaataaaa ctaatgaatt aataaataga gactcctttt ttttctgtta
      541 aataagacca acaagcaaat aatatttatt taatgagcta gaaataggag gagcttacgc
      601 agcataatac aagatattta ctggaaacct gtagcaaggt atgttattta acgttaacgt
      661 tgtaaatatt actttcactg ccacaaatga gatgagtgct tgtgattttt gtaaaagtta
      721 aatcccaaag aacaatttct gcttcccatg tagtctgtaa accctcacaa aacacaaaca
      781 gtagcaacaa caaaactctc tgagggatct gaagaatgaa caaaggcagc aagattctcg
      841 aagggggttg aagacacctg gaagtagtat aggttaaatt tcttatgact attatcctga
      901 ggtcaagcca caggtagggc cacacagagt ggctgaaact cctgtaggaa gctcagtctt
      961 tctggcctta tgaaccaggg gacagagttt ggagcaaccc cagctactgg aaagtaaggg
     1021 aggaatgtca ttaaaaagag ggccagaaaa ggagcattac aaatttttgg tgtaaattct
     1081 gctcaaatct ctggctgacc tttggaatat acatacacag gcagaccaaa gcagtcagtg
     1141 aatttgataa tatcaatata gattttctct atgaagaaca gagattgtat ttttaaaaag
     1201 gattaaaaaa aaagcaacca gagcctcagg ttcctatggg ataattcttt taacatatga
     1261 gtaattggtt ccccagaagg aaaggtgata gagaatgggt cataaaaata ttaggttggt
     1321 gcaaaagtaa tcgtggtttt gcaattactt ttcattacaa aacccacaat tacttttgtg
     1381 ccaacctaat atttgaagaa acagtggccc cacacacttc ccaaaattgg tgagagataa
     1441 atgtacacgt tcaagatgct gaggcacatt gtaatcaaac tgctgaaaac ccaagacact
     1501 atcttgaaaa tagccggaga aaaacaacac actatataca tgaaactgca ctgctaaata
     1561 ctgccaacct accttcaaaa actatgaaag caagaagaca atgaaacaat atcttcaatg
     1621 tgctgtaagg agataagact atccaatccc gaagtccata tccaaagata ctcatacttc
     1681 aggaacggag tcaaaataaa gttgcattca gaaaaacaaa aacagaatat ttattgccag
     1741 cagaccaact tacactgtaa aaaatgcttt gttctttggg atgacaagaa ataatagatg
     1801 aaaactcaga tccttatgta aaaataaaga aatcataaat agtaagataa atataaaaat
     1861 caattttttc ctcctaatgt tttaagaata tgtatgtttg tttaagtaaa aactatagca
     1921 tcatttttat gggatttata atacaggtag atgtaatttc aatgacaact tgaatttaga
     1981 gggagtttgt aaatagattc atacatgtgt gaggtttcca tattgtatgt aaatagtata
     2041 atagtaattc taggtagact gtaaaaagag aggttggatt gaattcctgg taatatgtgt
     2101 ttaaatggaa tgaatactcg ttcttagaat gaagagaagt gtgcagaatg gaactttgca
     2161 aacccttact ggcccatatg tcacggggtg gagatgtcac cattttctat atggctccag
     2221 atcaagcaat ctcttgacaa aaagcaagct ggcttcgagt aaccttcaaa cggagtgtac
     2281 caggccaatg cagaagacat aacatggttt gctttctttc tcccgtccat tttagtatct
     2341 taagtaccag cagatagaag gtccagaaaa aagaaagagg atgatattgt aggggaggcc
     2401 aaattttctc tcttagggtt ttttagctgg gcctgaggat tgaattgaca taaggcagat
     2461 cagcgggaga aaagcataca aatttaattc attcaatttt tatgtgagca caagagccct
     2521 cttaaggaaa tgaaacccaa agacagagtt gaaagttgaa cacctatgta ctgaattggg
     2581 aaaggaatag aaaattgtga gtatgtgaca aagccaacgg gcttgggcta gggaggttaa
     2641 ttgggtagag aagtaactag gaagataagg gttactttaa taaggtgtgt ttgttcagat
     2701 ttctctcagc atcaacttct cgtctttgat gataagaacg ctactttccc tctggcgtag
     2761 ggaggacaag ttatctcctg tttctaagga aaagaagaaa gctcagagta tattttttgt
     2821 atctgctgtt tttccaagtg tctttaagct caaaatagtc aatatactag agcaacatat
     2881 tttggagtat tgtgttctga actccttcaa tctttagcat ttctcttctg tcattcttca
     2941 ggctcttgag cacttttgag aaactaatat gaatgggaaa gatgtaatat ttactcccag
     3001 gaaggttgct ttggtgatct agtttgtgaa ctagactcag gaaactgtct gggtattgga
     3061 aaggaagtca cggatgcgca atggaatttg taaaggggtg gcacagctct tgaatactct
     3121 ccttggaaga gaccctggca tggagagaac agagtattaa cctcactctg aggttgggaa
     3181 gactaaggga atctgaggtg tgaggaggga tggagacgac aggtcacaca atgtgatgga
     3241 aaaccgcaca tggagtggaa agaacatgaa ctctggagtc tggaactcct atatctggac
     3301 ttgacaccgt attctgctat gtggccttcg gtgagtgtgc caagtagaat attggctctg
     3361 caaacgtatc cacatcaaat ttctagagct tgtgaatgtg accttgtttg caaaaaaaat
     3421 ggtccttgca gatatgatta aattgaggat cttgagatga ggaaatcatc ctggattatt
     3481 ttgggagacc taaatgctat tacaagagtc ccaccaacca ccagaagctg aaagtggcaa
     3541 ggaatggaat ccctcctaga gcctctggag ggatctttgc tcttaaacat cttaattttg
     3601 ggcttctggc ttccagagct gtgagagcca caaagtttgt ggttattttt tattgcagcc
     3661 acagtgaaga agataataaa gccagctact gacctctctg aatctcagtg ccctcatctg
     3721 taagatagaa tcagctagct actttatagc atttttccag gattaaatga gacatgtaaa
     3781 atagaaaaca aacaaacaag caaacaaaga aaagataagg aacatgctga gaaaaaaata
     3841 tagacacccc ttgtttgatg ttactaattg attcctgatg atcagatgta ttacccaaaa
     3901 atgatttcct tgagacagtt tgccattact taaatgggaa aaaatgtgtt atatgtcaaa
     3961 ccaaccctct agtgaatttc ttgaagtgta agtaaaacac agtaaaataa ccatagtaga
     4021 acaataatgt cacactgaga cttaaaatgc aggcatacct atatctcaga gatactgcat
     4081 gttcacttcc agaccatcac aataaagtga atattgcaat aaaacaaggc acacaaactt
     4141 ttttgtttcc cagtgaatat aaaagttatg tttacagtct atagtagtct agtatgcaat
     4201 agcattataa aaaaaatatg ttatcttaat ttaaaaattt tttttgctaa aaatactggt
     4261 gatcattttg aaaccctcca ggtaattcca taatcctttc ctgcctggtt ggaagggtcn
     4321 tttgcctttg atattttatt ggcttgccga actggtatca aactgggtgg ttgttgaagg
     4381 gcagaaatgg ctgtggcaat ttgtttaaag taagaaaaca gtaaagtttt caacatcagt
     4441 ggcttcttgc tttcacgaat gacttctctg ttgcatacag cactgtttgc cagcatttta
     4501 cccacaataa aactcctttc aaaatgagtg aatcctctcc aacccttctt tatcaactaa
     4561 gtttatgtaa tattctaaag cttttgttgt catttcaacg atgttcataa catcttcttc
     4621 aggagtagat tttacctcaa gaaatccttt tctttgctta cccgtaagaa gcaactcctc
     4681 actcattcaa gttttattgt aaaattacag taattcagtc acatcttcaa gctttacttc
     4741 tgattctagt tgtcgctatt tcttccacat cttcagctcc ttcctccact gaagtcttga
     4801 acccctcaaa gtcatccatg agagttggaa tcaacttctc ccaaacacct tttcatgtta
     4861 atattttgtt gtcctcccat aaatcatgaa tgttctcaat gacatccagc atggtgaatc
     4921 ctttccagaa ggttttcatt ttactttgcc cagatcaatc tgataaatca ctatgtatag
     4981 tcttacaaat gtattttctt aaataatgag aacttgaaag tcaaaattac tccttcaccc
     5041 atgggctgca gaatggatgc tgtgttggca ggcatgaaaa caacattcat ttccttttac
     5101 atctccatca gaactcttgg gtgaccaggt acattttcag tgagcagtaa tattttgaaa
     5161 ggaatctttt ttctgagcaa taggtctcaa gagagggctt aaaatattca gtaaaccata
     5221 ctataaacag atgtactgtc atctaggctt tcctgtttct tttcagagaa catgaagagt
     5281 tggtgtagta tcattcttaa gggccttagc attttcagaa tggtcaatga gcattggctt
     5341 caacttaaag tcaccagctg cgttagcagc taacaaaaga atcagcctgt tctttgaaga
     5401 tttgaagcca ggtatcgact tctcctctct agctaggaaa atcctagatg gcatcttctt
     5461 ccaacagagg gctgtttcat ctctcttgaa aatctgcctt tggccgggtg tggtggctta
     5521 cccctgtaat cccagcattg tgggaggccg aggcaggcaa ataatgaggt caggagttcg
     5581 agaccagcct agccaacatg gggaaaccat gtctctacta aaaatacaaa aaattagcag
     5641 ggcgtagtgg tgggcacctg taatcccagg tacttgggag gctgaggcag gagaattgct
     5701 tgaacccggg aggcagaggt tgcagtgagc tgagatcgca ccattgcact ccagccctag
     5761 tgacagagtg agactatgtt tcaaaaaaaa aaaaaaaata tgctgtttag tgtagccacc
     5821 ttcattagtt atcttagcta gatcttctgg ataacttgct gcagcatcta tataagcact
     5881 tcctgctttg ccttttatgt tctggagatg gcttctctcc ttgaacctca tgaatcagcc
     5941 tctgctatct tcaaactttt cttctgcagc ttctttacct cttttagcct tcatagaatt
     6001 gaatatagtt agggcctttc tctggtttaa gccttgggtt aagggaatgt tgtggctagt
     6061 ttgatcttct atccagacca ctcaaacttt cccatatcct caacaagcct gtttcacttt
     6121 cttatcattt gtatgttcat tggagtagca cttttaattt ccttcaagaa cttttccttt
     6181 gcattcacaa cttggctatt tggtgcaaga ggcctagctt tcatcctagc tcagcttttg
     6241 acatgccttc ctcgccaagc ttaatcattt ctaatttttg atctacaggg agagacatgg
     6301 gactcttcct ttcacttgaa cacttagaag ctgttgtctg cttattagtt ggactaattt
     6361 caatttgttg tgtctcaggg aatagggaag cctgaggaga gggaaaaatg gctagtcagt
     6421 gcagcagtca gaacacacaa acatttactg ataaagttca ccatcttata ttgggcccca
     6481 taacacttac aataggaaca tctaagatca ctgatcacag atcaccacaa cagacataat
     6541 aataatgaat tagtttgaaa tattgggaga attaccaaat tgtgatacat agacgtgaaa
     6601 tgaacacgtg ctgttggaaa aatggtgcca atagacttgc tagatgcaaa gttgccacaa
     6661 accttcaact tgtaaaacca cattatctgt gaagtgcaat gaaacaaagt gcagtaaaac
     6721 aaggtatgcc tgtacttaaa aaaattatat cctcgatcac ttgtacttaa aaaaattata
     6781 tccttgatga cttctacaat taacatgtta taaaatgttt gctttgtacg tagtagctat
     6841 tctcacttgt tttgttttct tctctacaat tgtcttcagt gttttctcaa cagtttgggg
     6901 atttcaacaa taagcaaaaa aagtaaaatg gggaaggcac tgggaaatgt gctctagtgc
     6961 ctggtggcca agtgtggctc acactgatgg tatgatggcc ttttagcatc agcaacttct
     7021 gaaatgggtg tttcgtgtgt tgtcactcag cttgctttcc aaaatatcta tatattattc
     7081 tgatgttcta ctgaatatgt tttgaaaata tcttgatgat tttcctatat ttccttttat
     7141 tgaaagtttt gcagagtttg gtattataaa tctaaagttt tgctacatga acattggcaa
     7201 aaaaaaaaaa aaatagatga tgaaatttgg attttaaaaa ccaagagtca ttttaccatg
     7261 aaaatgttaa tcgaggcaac atcattcagg ttatgcctgt atttgcagaa tgtttcatag
     7321 tagaaaatta atatgaaata taaaaagagt taatacaaac aagtaatcag aaataaaaat
     7381 caatagaaaa ataactaagg ggtctgaaga aacataaatt ggagaagcaa cagaaatgga
     7441 caattaatga atgaaagaat gtacaactgc aaaagtaatc aggaaatgcc ttctaaaatc
     7501 agcacagtat agctttttac atctatccaa ttgtcaaaat taaagggaaa aataaatcta
     7561 acaattctag gtgttggaga ggttgtaaag caatgaagat tatcattcac agagcaaact
     7621 ggtacagcca ctgtggaata tagtgtggca tcatccagaa tagttgaaga catgcaactc
     7681 ccatggccca catgccaagg ggaatgtgca caacatactt acaatagtat ctttttgata
     7741 attcaggaaa acgtacatgt tcaccaatag ctaatacata aatattatta ttcaactaag
     7801 agacgatttt cgaaaagcaa aaaaagaatg agtaaaaaca acatatagta acaagaataa
     7861 ctataaatat atgttgagta aagatgtttt gaaaatgagt ttcagaagaa tgcaatctaa
     7921 taccattata taacaattta aaacatgcaa aaaaaacccc atcattttca gctagttggc
     7981 aaatgtagtg gaattgggca tggggatatt ctccctagaa aaagaatttt ttcatgtttt
     8041 ttgatctagt aatctcattt atagaaatct ttcttaaaga aatagagatg caggcaaaaa
     8101 ttcattatta tactttggta ttatagtatt tatagaataa ttaagtactc taaattatct
     8161 gtcagtaaac tgtgaaattg ttaaataaat tatggaacat aaggtagtgt tttaaaatct
     8221 ttcaaaaatt atatctaata atatgggaaa attgcattgc tatgtaatcc gaatttggtt
     8281 taaaaattaa acaaatagca aaaaaagaag gcaaatatat aaataagact gggaactata
     8341 aaaacactat caatgtttat ctctgagtga tagaataata tgtgattttt aaaaatgttt
     8401 ttctgtaatt ctcaaattag gtgcagtaag cagacatgac ttctacaatc ataacaggta
     8461 gcttcattga cctgtttcta aaatgggtag cactttttaa ggattgaagt tgttttgtcc
     8521 agttggacag attcttatcc ctttgtcttt tcttgtattg ccaacttctg cctatacttg
     8581 aaatagtagg gcagatttct atatgtaagc atttaaaggg tctgcaagaa atactaagcc
     8641 atgtaggaac tgtgaatatt aaatgagata atgaatgaaa agaagcctag cacagtccct
     8701 agcccaagta agtacctatt attattatta tcattgactt tactacggct ttggtgttat
     8761 gtcacagaca aaagtttgca aaccactttt ttttttgctt gttttttttt tttaaggact
     8821 caaaacagtt acctttgagt actaaagtcc ttaagatgga aaacgagatg atatttatta
     8881 tgccactgat atgtttcagg aagagcttgt ggagagagca gacaaaacaa atggtaaatc
     8941 ttgttcttag aaagaaagag ctatctgtcc ccattacagc cagctgtcat gcccaggctg
     9001 gagccactct tgggacaaaa ttagggacac ttgaaaatgg taaatcttgg aaactgagag
     9061 ctctgtattt ttcaacttag gatcataatc tattaacccc tatttctatc ccagtttttc
     9121 tcttggataa ctgatttcat ttgtgcaact tgcattgaga aggtcaggat aaggcctgga
     9181 aattgtcaaa ctctagattt tgatgacatt ttggtaactc cttacaggaa aatctatcag
     9241 ttacatggtc caattgtagg ggtgatattt actaattttg cattctattg aacagcgata
     9301 tataatggaa tgtatgttgt gaatcttcaa taagtgtttc ttttctctta taacccaatg
     9361 aattgacctc tataataaca tgcagttgct aaatgaaaaa agaattgtat ttaagcagaa
     9421 aaaagtttta gaaaatgtga ttttgtatgc caaaatatgc atattagtag cttttatgag
     9481 aagtgtcctt gaaggaaaat atggtgaatt tgtatctggt tcaggtcttc ttttaataac
     9541 cagaaaacaa ggctgaaaca agcacaaggc agttctatct gtttgaatga tataaattgg
     9601 ttgttaaaaa taaaaataaa atgaaaatga aaaccaagat aacaatacca aggataagtt
     9661 aaacaaaaag ttggtttttt gaaaagatga acaaaattga taagcctctg gctgtactaa
     9721 ccaagaaaag atccaaataa aagtcagaaa tgaaaaagaa gacattacaa atgataccat
     9781 aggaatacaa aagatgatca gagactacta tgaagaagtg tacactcaca atctagataa
     9841 tgtagaggaa attgacaaat tcttgtaaac atatgactgc ccaagattga accagaaaga
     9901 aatagaaatc ttaaacagac caataatgaa tagtaagatt gaatcaataa ttttaaaacc
     9961 ttccccctaa aatgcccagg actggatggc ttcatagcca aattctacaa aatgtgcaaa
    10021 gaagaactga tactcatcct actgaaactc ttccaaaaaa atcaagatga ttaaataaag
    10081 aaaatgtgct ctgtgtgtgt atataagtgt atacatatgt atatatatac acacacacac
    10141 atacacatat acatatgtat acacatatat acacacacac cacatgtata tacatataca
    10201 catatataca tacagcacat tttatttatg tatatgtgtg tgtatatatg tatacataat
    10261 agaatactat tcagccacag aaaatgaatg aaaacatgtc ttttacaaca acatggatgg
    10321 aataggaggc cattatctta agtgaaaaaa ctcagaaaca tagtgaaata ccacatgtcc
    10381 tcacacgtgg gagctgaata atgtgtgtat gtagacatag agtatagaat aatagtcact
    10441 ggagactcag aatgatgata gggtgggagg ggagtgaggt agtacaatgt acactattta
    10501 ggtgttgatt acattaaaag cccagacttg accactatgc ggtatatcca tataacaaaa
    10561 ctgcacttgt acccctttaa tttacgtaat taaaaaattg gctgggcaca gtggctcatg
    10621 cctgtaatcc aaacactttg ggaggcccag gcgggcagat caattgagcc caggagttgg
    10681 agactagcct gggcaacatg gcaaaacacc acctctacca aaaaaataca aaaattagct
    10741 gggcatggtg ccacatgcct gtaatcccag ctactatgga ggctcaggtg agaggatccc
    10801 ttgaacccag gaggcggaag ttgtagtgag ccaagatggc gccacggcat tccagcctag
    10861 gtgacattat gagaccctgt ctcaaaaaca acaataacaa caataacaac aaaagaaaaa
    10921 attatgctca ctaaaagaaa aaaacagtaa ttaaaaaaaa tgagttcttg tggtgaaaca
    10981 taatggcacc tcctggttcc ttcttgcatt tgaagattat agatgaggat tttcagggaa
    11041 tggtcaagat caaaacctat ttggtctccc aagattttcc aggagaggct gtaggcctct
    11101 tcctagctta cctggcatgt acatactaac tgcaggtagc agctgtatga agttgttggg
    11161 taactcattt ttggttgtgt tctctaggtg acacattagg catttttgtc tacctttaag
    11221 tcctccgttc tcacctctgg tagagtgtaa actccataat gagagagact gcttctgact
    11281 tgtccacctc tgtaccctaa tttaatgaaa acatgttgaa caaataaaga ataaacctag
    11341 tggtggtccc aatcatccac ctgcccttcc aaccatcgtc ctttccagat tgcagccttc
    11401 tatatcagaa gtgtcagcct ttgttttctt gctctgggaa gtgatgattt aagttccaca
    11461 ccaccaaagg attgtcaaat acacacaaca aaaataaact tgagctaaaa ttgtgatcta
    11521 atatgcagag agattgaaaa agctaagaaa ataaattata ccactttggc catgcagata
    11581 caggaaccat ctggaagcat ctcagcaacc tgaaagggag catctgatgc ctcgaattgc
    11641 cctgtccgtc agctttagct gctattccca tttgcttctc ttaaatgtat ttaacttggg
    11701 acaagttggg agccaccttc tcttttctga ggtgatctta gaagattgat atacgtaagt
    11761 tgcagcttgt ttggattgtt tatctgtatt taaaggctct accaggcatt gtattttgct
    11821 gttaagataa aatcaataaa taaaaactta aaaaagaacc agactaatcc ttgaacattc
    11881 ctaaacatat actctctcct gctttcaaaa aggaacactg cttaatgttt acttattgta
    11941 gtcttctgag aatttgattt gctgtgattg cacctttatt caaaagtaca gaacctaaaa
    12001 atggtacaaa gaaagtttat ttcaaatcag atgcatttgc aagccagtgt cattaaaaaa
    12061 catgctgttg gtaaggctga ggtcagagtc tatcatacag acagatgtga tttattgcaa
    12121 aagagagccg actactgctt aggtatcagg tgccaccaat acataactac caccaacgaa
    12181 aggctggttg tgatgacggt attggaagct ggtgagtcag actcattaca attttcatgt
    12241 aatctctaaa cttccttgtt aaactatttt ctactcttcg taacttttga aaagagagaa
    12301 tatgggagaa cttgagcttc ctctttctcc ttctctggct tcttttgttc ccgggcagag
    12361 acattagcta gcagtcagat atctgagaac atggtaagag aaataagagc aggaagcctc
    12421 ggcaactcac ttgataatta tttttcatca tttagtagca ggtggtagaa taaataggat
    12481 catttgtaaa gttcaagtgt tgatggtggt agtggagaga gatactaaag cattttagtt
    12541 ttcaagccac aatgaagtaa ctggtatcat tcttgacctc atttcatgaa taactataaa
    12601 attagataaa atgtatgcgg caactgtttt cagaaattgg ctaacaggca atgcaggctg
    12661 tgacccttga gacaagtaaa cccctgaggc aagccttgca ttcacttggc tttctgccta
    12721 ggggtgcatt cactggactg gctagtaagg aagtggggcc aancattaca tgcttgtttc
    12781 aatgaactga taaggcagag tttgtacttt gttgctgctg aggggctgga attagcagag
    12841 tagggtactg aataagagag atgcatgagg taggcatgtg gaggtagtgt acatggaggt
    12901 ttctttgcag gtcattgtct gaaggattga ctatgtataa gcagggcaag acaccattaa
    12961 ggcctagcag agaaggttca ctgtgggacc aagagccgaa caaagacact agtggttgcc
    13021 cagagatggg agacgttgga attcttagtc tgagagggca gatttccctg agaacctggg
    13081 cattcagttg aaaccccaga aaggccagcc tcaggagtga gacctgtaca ctaccataag
    13141 gctgtgttct acaactaagg acaaaattga actagaccag ttctaacaat gaaaggttca
    13201 agagaatatt ttagaaatgt aatttcctgc cagaaaaaaa ctcagtaccc tataaggaag
    13261 acaatataat gcagattccc taaatgtgtc gccctgtgtt tagcatacaa caaaaaaatt
    13321 actggacgtg tgaagaggaa gctccattaa tcagaaaaaa gcaataaata gaaacagact
    13381 ttgaaataac tcagacatta gaattagaaa atgaggaatt taaaataact agataagtat
    13441 attcaaggac ttgacataaa atgggtgaca taaaatgaag aggcgggaaa tctcagagaa
    13501 acaaatgtaa actataataa agaaacaagt ctatattata gaactgcaaa taataatttc
    13561 caaaatgaaa agtttactga atggcttagc aacatattag aaactacaga aaagatcagg
    13621 aaactttaag acagatcagt agaaattgtc tgatctgaag aacaggagga aaaaatagaa
    13681 aaaatatatg aatcttcagt gatctgtggg gcaaaagcga gaagtctaac atttgtacaa
    13741 tttgaggtcc agaaatgagg atagagagaa tgagggagga aaaaaaatag tttaaaaaaa
    13801 tgaacaaatt ttcccaaata tagtgaaaaa atcaaactac aggttgaaga tgtttagcaa
    13861 accaaagcag gataaaaaca aagaaaacca tacctggatg tatgacggcc aactgataaa
    13921 aatccaaaag taaagaagaa agctgctgag gatgggagga gtggagagac attacatata
    13981 ggagaacaac agtaagagtt attttagact cctaaataaa aactattcaa gccagaagac
    14041 aatagaatga catttttaaa ttcttttttt aaatttaaat ttattttatt ttaaattctg
    14101 ggaaacgtgt gcaggatgtg tagctttgtt acatatgtaa acatttgcca tggtggtttg
    14161 ctgtacctat caaaccatca cctaggtatt aagccacaca tgcattagct atttatcctg
    14221 atgctctccc tcccaccgct gccccccatc ccaccccaga caggccccag tgtgtgttgt
    14281 ttccctccct gtggccatgt gttctcattg gtcagctccc acttatgagt gagaacatgc
    14341 agtgtttggt tttctgttcc tgtgttagct tgttgaggat gatctcttcc agctctatcc
    14401 atgcccctgc aaaggacatg atctaattcc tttttatggc tgcataaaca aaacctaagt
    14461 tttagataaa caaaacctaa gaaaatgtac tgctagtcaa cccgcattat aagaaatgtt
    14521 aaagcaaatt cttcagccta aaagaaagtg aaagcaaatg gaaacagatt aacaggaagg
    14581 aatgaggagc acagaaatgg taaatatatg gaataatata tatattttta aactgtcttt
    14641 attttctgtt aagctcttac ggggtaactt actatttaaa gcaaaactca tgacaatgta
    14701 ttgaaaggat tataatgcaa atagaagtaa aacatgtgac agcataatgg acaaggaagt
    14761 aataaatgtc aggttcttat attttacatg agatagtata atattaattt taggtagatt
    14821 ttgataagat aaggatgcat attgttagtc ctagagcatg cacgcacaca cacacacaca
    14881 cacacacaca cacacacaca cacacacaca cacacacatg ctacaaagag gtataattaa
    14941 aaaccctaat agtggtataa agatggaaac tttagaaata tgcattttaa cagaaagaag
    15001 gcaagaaaga aggaagaagg aaacaaaaaa agatggcata aatattcaga tggtagactt
    15061 aaaactgaat tatatcatta cattaaatat aaatggacta aatgtcccaa ttaatagtca
    15121 tagattgtca atatggatac tgtcaggcct ctgagcccaa gccaagccat cgcatcccct
    15181 gtgacttgca cgtatacgcc cagatggccc gaagtaactg aagaatcaca aaagaagtga
    15241 atatgccctg cctcacctta actgatgaca ttccatcaca aaagaagtgt aaatggctgg
    15301 tccttgcctt aagtgatgac attaccttgt gaaagtcctt ttcctagctc atcctggctc
    15361 aaaaagcacc cccactgagc accttgcgac ccccactcct gccagccaga gaacaaaccc
    15421 cctttgactg taattttcct ttacctaccc aaatcctata aaatggcccc acccttatct
    15481 cccttcgctg actctctttt tggactcagc ccgcctgcac ccaggtgaaa taaacagcca
    15541 tgttgctcac acaaagcctg tttggtggtc tcttcacacg gacgtgcatg aaagatacaa
    15601 aataaataaa acccaataat atgctgacta caacatatgc actttaaata caaaaacaca
    15661 aagagttaaa agtaaaagga atacaaatat atgttataga aataattttt agaaagccaa
    15721 tgtggctaca tcaattacac aaagtaaact ttaagacaag aaggtttacc aaagataaag
    15781 agggacatat cataatgata aaggggtcaa ttaagaacaa ataataattc caattttttt
    15841 ttcaagacag agttttgctc ttgttgccca ggatggagtg caatggcatg gtctcagctc
    15901 cctgcaatct tcttctcctg gtttcaagtg attctgctgc ctcagcctgc caagtagctg
    15961 ggattacagg tgcctgccac tacacccggc taatttttgt gttttttttt tttttttagt
    16021 agagatgggg tttcaccatg ttggtcaggc tggtcacgaa ctcctgacct cagctgatcc
    16081 acttgcctca gcctcccaaa atgctgggat tacaggcatg agccactgca cccggccaat
    16141 aattctaaat gtttactcaa ctaaaacttt aagaactttc agatatagga atcaagagcg
    16201 ggattttttt ttcaatattg tattatgaaa aaattaaacg tatatgaaaa ctgaagtaat
    16261 tttatagtga acaccctgtg taaccaccgc ttggtgtcta acattaacat tctactgtgt
    16321 ttattatatt acatttgtac ctatctgtta ccccttcttt ctatttgtca attcatcagc
    16381 tgcagtgatt ttgaaactct actctgtaaa gttccaggaa ttgtgtgtgt gtgtgtatgt
    16441 gtgtattttg gaagtggggt agtgttttga gaacactagt agtcctcatt tgaccaaata
    16501 atttatactt ttatctatta ttctatctta tatattggac ttctgtttag gttacctgaa
    16561 aacatggttt tgtgagtaaa ggaaaatttt gaaagctttt atcaagggta ttctgagggt
    16621 aatccactct cccaatctag ttaagcataa tctagaaacc tgaccatgtg ttccttgaag
    16681 acactgagtg agtgtaccca atttggcagc ttttgtgaga gcaaggacca tccttcattt
    16741 ttaattccct tcaggttcat gcctacagtg aaaagtcagt acatcttgct ggatgaatga
    16801 aggtgggcaa tgtatttatt ctatataata ttgtattttg tgggcacacc tgtgagaata
    16861 tgacacaggt tggacagttc tacctggctc ttagctatgt taattaccaa ctaattatga
    16921 ggttgtttta ttttcagaac cccgttaagt ccatttatta attagaataa gactataaat
    16981 tccctctgtt taatccaaat ttcactatct ggcatccccc aaatcagaaa tgttaacact
    17041 ggaacattgc ttgctttcaa attaaatagg taagatttga agagaacttt ggagtttaag
    17101 aggttacatc taatgtgttc taaatattag gactcgtttt tcttctgatt acacccctac
    17161 aaagtgccaa gcctttgata gctcattttg tgttttctga agttctagtt ctcctggtca
    17221 cattctccag caaggccctg tgtattaagt gtaaccagta atggaaaatc ctctacatag
    17281 aatgtactca gaaaatttga ggagcttaag cacttgactt ctctttggaa acacagtgga
    17341 tatttgtgtc gctactgtac acaaaggagg agacactgag aaataaagaa ggttatccca
    17401 agtccccagt aaaacaggat gaggttttta ggtttgagga aagtgcctca ttcactccat
    17461 tcctgcctca ggatactgaa aggcaggttt ctaggctgag ttttctactg taggaaatcc
    17521 tattttttac ttatcaagaa tgcattagag ttggaagaat tacagtcttc ctacaccata
    17581 tgctaatgtt aattccagct ggattaaagc aataaataaa tgtataaaat caatcattag
    17641 aaacccagaa tagaaatgag tatttatccc tactggagtg agaggggact tcataaactt
    17701 gaagacaaag agaaagatcg tttctgaaaa gagtaataga tttgactcca ttaaaaatta
    17761 aaacttctgt ttgccaaaaa gcaaatccac acagtgataa gtatgtgcca cagatatgag
    17821 ggacaagtga ctaggaatct taaaagacca agccaattga taacaatgca tgattacaat
    17881 ggttaaatgg gaggtcaggc atccattgtt ttcctgccta atgctgttat tttcaattct
    17941 ttcttgctca gtgggagaac tcaattgccc agggtctctt cctgcttgct tttctatcat
    18001 tcctatttct gagccccagc aggggctggg ttcttcttct cttcttcttc cttcttcttc
    18061 ttcttcttct cttcttcttc cttcttcttc cttcttcttc cttcttcttc cttcttcttc
    18121 ttcttcttct cttcttcttc cttcttcttc cttcttcttc cttcttcttc cttcttcttc
    18181 ttcttcttct cttcttcttc cttcttcttc cttcttcttc cttcttcttc cttcttcttc
    18241 ttcttcttct cttcttcttc cttcttcttc cttcttcttc cttcttcttc cttcttcttc
    18301 ttcttcttct cttcttcttc cttcttcttc cttcttcttc cttcttcttc cttcttcttc
    18361 ttcttcttct cttcttcttc cttcttcttc cttcttcttc cttcttcttc cttcttcttc
    18421 ttcttcttct cttcttcttc cttcttcttc cttcttcttc cttcttcttc cttcttcttc
    18481 ttcttcttct cttcttcttc cttcttcttc cttcttcttc cttcttcttc cttcttcttc
    18541 ttcttcttct cttcttcttc cttcttcttc cttcttcttc cttcttcttc cttcttcttc
    18601 ttcttcttct cttcttcttc cttcttcttc cttctacatt tccatgggca tttcccctgc
    18661 ctggagggct ttctagctct cacatgcaat cttcagtgat gttttcgtga ggtctatttg
    18721 gggattccca gcaatgttgt tctgtgccat ttgttattag gtatgggtgt tttatgctca
    18781 tctttcattg gacaaatgat tgttttattt ctagaaaaga aaattcatca tgttccccta
    18841 cttcctatct tggatgcttg aaacatacat gtatgtgtat gttagtgtgt atatatgtgt
    18901 gtgtttgtgt gttcaaggtt ttaagctaga agcgggtaat ggctttagga agtaatggtg
    18961 gggaaatctg tcacagcatt atcacatgtt cccatctgct gaaagcagaa aaatgattgt
    19021 gagattgtga tgaccagagg gaagtgatga ccaagtgagc ttatgttctg aaccaggttt
    19081 ctgtaaatca cattaagcaa gtttctccct gttactccta tgtccatttg caaggatgaa
    10141 cacttataag tcatgtttac attctaccaa caaccactct tttaacaggt aggtcttgtt
    10201 ccattgtaca gccccagaga aaatatgcat gaacattcat tcatgtataa gtacctttaa
    10261 ggaagaatct gctttgttca aggcactgta aaatacacaa agataaatta ccaagatgtt
    10321 tcaaatccgt ggatgtacac aaataattat aataagatcg agtcgactcc tttagtgagg
    10381 gttaattgag ctcgcggccg cgagctctaa

 

This sequence has undergone a major mutational event.  Please identify the following:

What type of mutation is this?

What is the name of the original locus?

What types of mutational events are commonly associated with this locus? (How does this relate to your answer to question # 6)

What other sequence(s) are involved with this locus (that might not have been involved before the mutation event....)
 
 
 
 
 
 
 
 


 You have been asked to help piece together what has happened at the following crime scene: In the past 3 days, about 28,000 people in New York city have suddenly died of apparent bacterial infection.  Police have localised the contamination to one of the subway stations.  Inspection of some broken glass from the rail track shows the apparent source: a light bulb has been cut open and filled with freeze-dried bacteria, and then taped to the rail.  When the train came through, breaking the glass, everyone was exposed to the highly contageous bacteria.  You have managed to sequence some of the toxin, and come up with the following bit of amino acid sequence:

MTEVLRNSPYRCDISSLNSPDAEWRKVIFKAIDESLSKKMGRTQ
 
 Based on this amino acid sequence, what do you think the source of the toxin was?  Why would this be so bad?  How does this toxin work?
 
 
 
 
 
You instruct your research team to try and design some PCR primers, based on the sequence from problem #8, and eventually, after several weeks of sleepless nights, come up with the following sequence:
 
        1 gttaactgtg gtggttgtca ccgcccatta cacggcatac agctatatcg agccttttgt
       61 acaaaacatt gcgggattca gcgccaactt tgccacggca ttactgttat tactcggtgg
      121 tgcgggcatt attggcagcg tgattttcgg taaactgggt aatcagtatg cgtctgcgtt
      181 ggtgagtacg gcgattgcgc tgttgctggt gtgcctggca ttgctgttac ctgcggcgaa
      241 cagtgaaata cacctcgggg tgctgagtat tttctggggg atcgcgatga tgatcatcgg
      301 gcttggtatg caggttaaag tgctggcgct ggcaccagat gctaccgacg tcgcgatggc
      361 gctattctcc ggcatattta atattggaat cggggcgggt gcgttggtag gtaatcaggt
      421 gagtttgcac tggtcaatgt cgatgattgg ttatgtgggc gcggtgcctg cttttgccgc
      481 gttaatttgg tcaatcatta tatttcgccg ctggccagtg acactcgaag aacagacgca
      541 atagttgaaa ggcccattcg ggcctttttt aatggtacgt tttaatgatt tccaggatgc
      601 cgttaataat aaactgcaca cccatacata ccagcaggaa tcccatcaga cgggagatcg
      661 cttcaatgcc acccttgccc accagccgca taattgcgcc ggagctgcgt aggcttcccc
      721 acaaaataac cgccaccagg aaaaagatca gcggcggcgc aaccatcagt acccaatcag
      781 cgaaggttga actctgacgc actgtggacg ccgagctaat aatcatcgct atggttcccg
      841 gaccggcagt acttggcatt gccagcggca caaaggcaat attggcactg ggttcatctt
      901 ccagctcttc cgacttgctt ttcgcctccg gtgaatcaat cgctttctgt tgcggaaaga
      961 gcatccgaaa accgataaac gcgacgatta agccgcctgc aattcgcaga ccgggaatcg
     1021 aaatgccaaa tgtatccatc accagttgcc cggcgtaata cgccaccatc atgatggcaa
     1081 atacgtacac cgaggccatc aacgactgac gattacgttc ggcactgttc atgttgcctg
     1141 ccaggccaag aaataacgcg acagttgtta atgggttagc taacggcagc aacaccacca
     1201 gccccaggcc aattgcttta aacaaatcta acattggtgg ttgttatcct gtgtatctgg
     1261 gttatcagcg aaaagtataa ggggtaaaca aggataaagt gtcactcttt agctagcctt
     1321 gcatcgcatt gaacaaaact tgaaccgatt tagcaaaacg tggcatcggt caattcattc
     1381 atttgactta tacttgcctg ggcaatatta tcccctgcaa ctaattactt gccagggcaa
     1441 ctaatgtgaa aagtaccagc gatctgttca atgaaattat tccattgggt cgcttaatcc
     1501 atatggttaa tcagaagaaa gatcgcctgc ttaacgagta tctgtctccg ctggatatta
     1561 ccgcggcaca gtttaaggtg ctctgctcta tccgctgcgc ggcgtgtatt actccggttg
     1621 aactgaaaaa ggtattgtcg gtcgacctgg gagcactgac ccgtatgctg gatcgcctgg
     1681 tctgtaaagg ctgggtggaa aggttgccga acccgaatga caagcgcggc gtactggtaa
     1741 aacttaccac cggcggcgcg gcaatatgtg aacaatgcca tcaattagtt ggccaggacc
     1801 tgcaccaaga attaacaaaa aacctgacgg cggacgaagt ggcaacactt gagtatttgc
     1861 ttaagaaagt cctgccgtaa acaaaaaaga ggtatgacga tgtccagacg caatactgac
     1921 gctattacca ttcatagcat tttggactgg atcgaggaca acctggaatc gccactgtca
     1981 ctggagaaag tgtcagagcg ttcgggttac tccaaatggc acctgcaacg gatgtttaaa
     2041 aaagaaaccg gtcattcatt aggccaatac atccgcagcc gtaagatgac ggaaatcgcg
     2101 caaaagctga aggaaagtaa cgagccgata ctctatctgg cagaacgata tggcttcgag
     2161 tcgcaacaaa ctctgacccg aaccttcaaa aattactttg atgttccgcc gcataaatac
     2221 cggatgacca atatgcaggg cgaatcgcgc tttttacatc cattaaatca ttacaacagc
     2281 tagttgaaaa cgtgacaacg tcactgaggc aatcatgaaa ccactttcat ccgcaatagc
     2341 agctgcgctt attctctttt ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt
     2401 tacttcttgt gccaatgtcg tggttgttcc cccatcgcag gaacacccac cgtttgattt
     2461 aaatcacatg ggtactggca gtgataagtc ggatgcgctc ggcgtgccct attataatca
     2521 acacgctatg tagtttgttc tggccccgac atctcggggc ttattaactt cccaccttta
     2581 ccgctttacg ccaccgcaag ccaaatacat tgatatacag cccggtcata atgagcaccg
     2641 cacctaaaaa ttgcagaccc gttaagcgtt catccaacaa tagtgccgca cttgccagtc
     2701 ctactacggg caccagtaac gataacggtg caacccgcca ggtttcatag cgtcccagta
     2761 acgtccccca gatcccataa ccaacaattg tcgccacaaa cgccagatac atcagagaca
     2821 agatggtggt catatcgata gtaaccagac tgtgaatcat ggttgcggaa ccatcgagaa
     2881 tcagcgaggc aacaaagaag ggaatgattg ggattaaagc gctccagatt accagcgaca
     2941 tcaccgccgg acgcgttgag tgcgacatga tctttttatt gaagatgttg ccacacgccc
     3001 aactaaatgc tgccgccagg gtcaacataa agccgagcat cgccacatgc tgaccgttca
     3061 gactatcttc gattaacacc agtacgccaa aaatcgctaa ggcgatcccc gccaattgtt
     3121 tgccatgcag tcgctccccg aaagtaaacg cgccaagcat gatagtaaaa aacgcctgtg
     3181 cctgtaacac cagcgaagcc agtccagcag gcataccgaa gttaatggca caaaaaagaa
     3241 aagcaaactg cgcaaaactg atggttaatc cataccccag cagcaaattc agtggtactt
     3301 tcggtcgtgc gacaaaaaag atagccggaa aagcgaccag cataaagcgc aaaccggcca
     3361 gcatcagcgt ggcatgttat gaagccccac tttgatgacc acaaaattta gcccccatac
     3421 gaccactacc agtagcgcca acaccccatc ttttcgcgac attctaccgc ctctgaattt
     3481 catcttttgt aagcaatcaa cttagctgaa tttacttttc tttaacagtt gattcgttag
     3541 tcgccggtta cgacggcatt aatgcgcaaa taagtcgcta tacttcggat ttttgccatg
     3601 ctatttcttt acatctctaa aacaaaacat aacgaaacgc actgccggac agacaaatga
     3661 acttatccct acgacgctct accagcgccc ttcttgcctc gtcgttgtta ttaaccatcg
     3721 gacgcggcgc taccgtgcca tttatgacca tttacttgag tcgccagtac agcctgagtg
     3781 tcgatctaat cggttatgcg atgacaattg cgctcactat tggcgtcgtt tttagcctcg
     3841 gttttggtat cctggcggat aagttcgaca agaaacgcta tatgttactg gcaattaccg
     3901 ccttcgccag cggttttatt gccattactt tagtgaataa cgtgacgctg gttgtgctct
     3961 tttttgccct cattaactgc gcctattctg tttttgctac cgtgctgaaa gcctggtttg
     4021 ccgacaatct ttcgtccacc agcaaaacga aaatcttctc aatcaactac accatgctaa
     4081 acattggctg accatcggtc cgccgctcgg cacgctgttg gtaatgcaga gcatcaatct
     4141 gcccttctgg ctggcagcta tctgttccgc gtttcccatg cttttcattc aaatttgggt
     4201 aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt
     4261 attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt cttttgtaag
     4321 cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg gggattttgc
     4381 cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta cgttgcaata
     4441 ttccgtgggc cgccgactta acccggctaa catccgcgcg ctgatgacag caggcaccct
     4501 ctgtttcgtc atcggtctgg tcggttttat tttttccggc aacagcctgc tattgtgggg
     4561 tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg agtatatgtt
     4621 gattgaccat attgcgccgc cagaaatgaa agccagctat ttttccgccc agtctttagg
     4681 ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca gcctgccgcc
     4741 ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc tgatgttaaa
     4801 agggattcga gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt cgaacacaat
     4861 aaagatttaa ttcagccttc gtttaggtta cctctgctaa tatctttctc attgagatga
     4921 aaattaaggt aagcgaggaa acacaccaca ccataaacgg aggcaaataa tgctgggtaa
     4981 tatgaatgtt tttatggccg tactgggaat aattttattt tctggttttc tggccgcgta
     5041 tttcagccac aaatgggatg actaatgaac ggagataatc cctcacctaa ccggcccctt
     5101 gttacagttg tgtacaaggg gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac
     5161 cggcggtgaa tgcttgcatg gatagatttg tgttttgctt ttacgctaac aggcattttc
     5221 ctgcactgat aacgaatcgt tgacacagta gcatcagttt tctcaatgaa tgttaaacgg
     5281 agcttaaact cggttaatca cattttgttc gtcaataaac atgcagcgat ttcttccggt
     5341 ttgcttaccc tcatacattg cccggtccgc tcttccaatg accacatcca gaggctcttc
     5401 aggaaatgcg cgactcacac ctgctgtcac ggtaatgttg atatgccctt cagaatgtgt
     5461 gatggcatgg ttatcgacta actggcaaat tctgacacct gcacgacatg cttcttcatc
     5521 attagccgct ttgacaataa tgataaattc ttcgcccccg tagcgataaa ccgtttcgta
     5581 atcacgcgtc caactggcta agtaagttgc cagggtgcgt aatactacat cgccgattaa
     5641 atgcccgtag tatcattaac caatttaaat cggtcaatat ccaacaacat taaataaaga
     5701 ttcagaggct cagcgttgcg taactgatga tcaaaggatt catcaagaac ccgacgaccc
     5761 ggcaatcccg tcaaaacatc catattgcta cggatcgtca gcaaataaat tttgtaatcg
     5821 gttaatgccg cagtaaaaga aagcaacccc tcctgaaagg cgtcgaaatg cgcgtcctgc
     5881 cagtgatttt caacaatagc cagcattaat tcccgaccac agttatgcat atgttgatgg
     5941 gcagaatcca ttagccgaac gtaaggtaat tcatcgttat cgagtggccc cagatgatca
     6001 atccaccgac caaactggca cagtccataa gaatggttat ccgttatttc tggcttactg
     6061 gcatctctcg cgaccacgct gtgaaacata ctcaccagcc actggtagtg ggcatcgata
     6121 gccttattga gatttaacaa gatggcatca atttccgttg tcttcttgat cattgccact
     6181 cctttttcac agttccttgt gcgcgctatt ctaacgagag aaaagcaaaa ttacgtcaat
     6241 attttcatag aaatccgaag ttatgagtca tctctgagat aacattgtga tttaaaacaa
     6301 aatcagcgga taaaaaagtg tttaattctg taaattacct ctgcattatc gtaaataaaa
     6361 ggatgacaaa tagcataacc caatacccta atggcccagt agttcaggcc atcaggctaa
     6421 tttattttta tttctgcaaa tgagtgaccc gaacgacggc cggcgcgctt ttcttatcca
     6481 gactgccact aatgttgatc atctggtccg gctgaacttc tcgtccatca aagacggccg
     6541 caggaataac gacattaatt tcaccgctct tatcgcgaaa aacgtaacgg tcctctcctt
     6601 tgtgagaaat caaattaccg cgtagtgaaa ccgaagcgcc atcgtgcatg gtttttgcga
     6661 aatcaacggt catttttttt gcatcatcgg ttccgcgata gccatcttct attgcatgag
     6721 gcggcggtgg cgctgcatcc tgttttaaac cgccctggtc atctgccaac gcataaggca
     6781 tgacaagaaa acttgctaat acaatggcct gaaatttcat actaactcct taattgcgtt
     6841 tggtttgact tattaagtct ggttgctatt tttataattg ccaaataaga atattgccaa
     6901 ttgttataag gcatttaaaa tcagccaact agctgtcaaa tatacagaga atttaactca
     6961 ctaaagttaa gaagattgaa aagtcttaaa catattttca gaataatcgg atttatatgt
     7021 ttgaaaatta ttatattgga cgagcataca gaaaaagcaa atcaccttta catataaaag
     7081 cgtggacaaa aaacagtgaa cattaataga gataaaattg tacaacttgt agataccgat
     7141 actattgaaa acctgacatc cgcgttgagt caaagactta tcgcggatca attacgctta
     7201 actaccgccg aatcatgcac cggcggtaag ttggctagcg ccctgtgtgc agctgaagat
     7261 acacccaaat tttacggtgc aggctttgtt actttcaccg atcaggcaaa gatgaaaatc
     7321 ctcagcgtaa gccagcaatc tcttgaacga tattctgcgg tgagtgagaa agtggcagca
     7381 gaaatggcaa ccggtgccat agagcgtgcg gatgctgatg tcagtattgc cattaccggc
     7441 tacggcggac cggagggcgg tgaagatggt acgccagcgg gtaccgtctg gtttgcgtgg
     7501 catattaaag gccagaacta cactgcggtt atgcattttg ctggcgactg cgaaacggta
     7561 ttagctttag cggtgaggtt tgccctcgcc cagctgctgc aattactgct ataaccaggc
     7621 tggcctggcg atatctcagg ccagccattg gtggtgttta tatgttcaag ccacgatgtt
     7681 gcagcatcgg cataatctta ggtgccttac cgcgccattg tcgatacagg cgttccagat
     7741 cttcgctgtt acctctggaa aggatcgcct cgcgaaaacg cagcccattt tcacgcgtta
     7801 atccgccctg ctcaacaaac cactgataac catcatcggc caacatttgc gtccacagat
     7861 aagcgtaata acctgcaggc actaacgtca aaaattctag gagggcatga acggcatgta
     7921 ggatgcccca tttggtaaag aaatcatgca gcaatcttac ttacggcaag aggagtttgc
     7981 aggggcaatg actgaagtac tgcggaattc gccttatcgc tgtgatatat catcacttaa
     8041 tagccccgat gcagagtggc gaaaagtgat ttttaaggcc attgatgaat ccttatcgaa
     8101 aaaaatgggg cgaactcagc caactcagta taggtttctt ttcggccaat ctccaacagt
     8161 atttatgaat gggttatctg ctgcaacaaa tggctcccct gactttgttg cttttaaatc
     8221 agagttaatt cagctaatta aggagcgagg acaatattgg gaaaaaatgc ctgaaatttg
     8281 gctaggccgt ttctttagaa tagaagaagg gttggctaca tcatttatga gaaacgtgta
     8341 tcctgatttc ccaccaatca acgatacaag aatgacatgg aatcacacaa aaataatggc
     8401 ctcagatggt actgaagctc ttgttggtgg acataacatg aacatggatc tatttagaaa
     8461 ttatccacct gttcatgatg tatcaattat cactcatggt tcttctgctt atggctccca
     8521 gctatatctt aacgaactat ggtcatgtaa ttcagattta ctaaaaaaag aatattttga
     8581 ttatgaaagc atgatgtggg cggtcggaac aaagttctat gataagcctg aagatccgct
     8641 taaaagctca gttgctatga attatatgaa gcaacggcaa gaggacctac tcaacttgca
     8701 tgaaaacttt aatcagaagg tagcgactcg tattagtgaa tacgaaaaca tggaagagta
     8761 taaaaaagca gacagagttt tatcagtagg taaatattgg acaggaccta atatggagca
     8821 tgactaccaa agagggtctg aaataatgaa agagcaactg ataaaaaatg ctaagcgcat
     8881 aattagaatt tcacagcaag atctcgtgag tgcttggaaa aaaaaatgga aagaccactt
     8941 tacgtgtaat tggattattg aggctttgtt agaaaataaa gatcttcata ttcatgttgt
     9001 agtctctgct ctagatgcag cagctggagc tggtgatcag tactcatttg gttctggagc
     9061 agaacggacc tatgaattat ttaagtatta cctaacccat gatattgata ccgatgaagt
     9121 attagacgat cctgatggta gccgtgctga tgccttaaaa agaatattga ttgcaccatt
     9181 cttctttaca gataaagtac ctgatgaaaa tacaattgaa ggcgaaacct acaagtggcc
     9241 tgatttagaa caaagcgctt atactgcaac acttaagcaa aaaccacttt cggaaaaacc
     9301 cccgcatcaa ggtattattg gtagtgcact aatgtcagca attaaaggta gtggactttt
     9361 ctatcctaaa gtccctgttg cacctggtaa tcacgccaaa ttaatgatta ttgacgatga
     9421 gttgtacgtt gttggatcag ataatcttta tcccggttat ctgtcagagt ttgactattt
     9481 agtcgaagga aaagatgcag ttaatgaatt aatgaaatct tactgggaac cattgtggaa
     9541 atattctagc ccacatgcat ttccaaaatt aagcccaaat tgatgactag aatacactag
     9601 taagtaataa tcagtcataa atcacataaa ccatcatgct aataatcatg atggttatca
     9661 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     9721 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     9781 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     9841 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcccggg
     9901 gatccgtcga cctgcagcca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
     9961 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    10021 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    10081 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
    10141 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
    10201 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
    10261 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    10321 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
    10381 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10441 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10501 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    10561 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    10621 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10681 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    10721 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    10781 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    10841 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    10901 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    10961 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    11001 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    11061 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    11121 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    11181 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    11241 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    11321 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    11381 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11421 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11481 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    11541 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    11601 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    11661 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    11721 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    11781 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    11841 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    11901 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    11961 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    12021 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    12081 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    12141 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    12201 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    12261 agtcagtgag cgaggaagcg gaaga
 
 

What does this sequence tell you, in terms of possible mechanisms of how this bacteria might have come about??  Do you think it is reasonable that this is just an "accident" of nature, or do you think it is likely that this was engineered by a person using standard recombinant DNA methodologies?
 

 
 

You have the following sequence cloned into a plasmid, in Escherichia coli;  however, you seem to have a difficult time in keeping this clone stable.  Please explain what types of problems might explain the instability.

        1 ttaatgatta ttatgatgta aagttaaaag aatctcggtg atgctgccaa cttactgatt
       61 tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc
      121 ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta
      181 tctctgctct cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc
      241 cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaactgc
      301 ccgcattatg ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag
      361 tcggtaacct cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa
      421 cagtggggct atgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagt
      481 ctccggaaga cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt
      541 cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg
      601 tatgaatccc gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcggcgaatt
      661 gagcggcata acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg
      721 ttctaaaaat cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac
      781 tatcaataag ttggagtcat tacccctggc gtccagcaca tcacgcgact agatgggcat
      841 cttattcttc tttgcttcaa tcctagagct tcttccagag aggatttaaa gtccatcatc
      901 tcaaccagtg agttgtagcc aaaggatgca gtgtgcaaat agtggtcggc atctcgtggc
      961 gatgagatta cgtaggtgta ccagtttgtc agcccctaag agcaagagtg ttcagtccgt
     1001 tctacataag tgccgatttt acactggctt gcggttggga tcacttgctt tgccgcgtca
     1061 tgtccgccag acgtgaggtg aaggacgttt tttctggcaa aataaccgtg gcaaaaggtg
     1121 ataaataaca acatttctca tttgactgtt agcatccgaa taataacttt gtgagcggtc
     1181 ggtggtttag cgattcgtgg cgatacccta aaggcagcca ataccctccg ggacggcgta
     1241 attttgctta aagttatatt tttcgacagc ggaacattta gcaaatgggc aaaaatcgcg
     1301 ccatgctttc cttcgttttg tcttttatat catagactta tttttgttta atgaatgttt
     1361 aagatatgta ttatttgagt aatctattaa tcagtaggct tttttatttt tattcccggc
     1421 gtccagcgct ataatctgcg gacgattttc tttgtggaat gtgcattgta gagcatgagt
     1481 cgtttatccc acctgtcgga acatagcgcc gccgccgtgt gcaaagggcc atcaagcctg
     1541 aacgcgcgcg gtcttcctgg cggtgaagac gatgtctctt cttatctttc cccacgtcgt
     1601 aaacgctcct ttctgacggt tgccagcatc ggctgggcgc ccgttgcgct tttttcggtg
     1661 gcggtaaggg catgcgtttt gccctgcgta attgagaaag tcctgatggt taacccggcg
     1721 gcaaatgcgg aaagtgtgat gcgtttggcg gccatgtgag ggaagataca tgttaaaagc
 

Single-letter abbreviations of amino acids

 
The Genetic Code
as used by nearly all eukaryotic nuclear genomes


 

Mitochondrial Genetic Code
as used by the human mitochondrial genome
 
 
 
 
To get a nice, handy-dandy box like this:
 
 
use the following web site:
http://www.ncbi.nlm.nih.gov/cgi-bin/BLAST/nph-newblast?Jform=0
 

To use this, all you have to do is paste in your sequence, and then click on the particular line of interest.  In this case, the RED lines are the best matches.  Notice that you have an insertion (hint, hint) into the middle of the gene.  The red line on the next line is the "insert" (likely a transposable element of some sort).  Please try this out and hopefully you can get it to work....
 
 
 

 

http://www.ncbi.nlm.nih.gov/PubMed/
 
 
 
 
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