100 points Total, 10 points per question.

Lecture # 9 - "The Double Helix" by James Watson

1. a. (5 pts.) In their paper reporting the structure of the B-DNA helix, (Nature, 1953), Watson & Crick state that knowledge of the structure of the double helix "suggests an obvious means of replication".  What did they mean by this?  Were they correct in their general  idea of how DNA replicates?
 
 
 

1. b. (5 pts.) What does the hydrophobicity of nucleotide bases have to do with the type of structure formed by DNA and RNA?
 
 
 
 
Lecture # 10 -  Chapter 5a - The Structures of DNA

2. (10 points) Give an example of (e.g., a short DNA sequence) for each of the three families of DNA helices.  Which type is most common?
 

 

Lecture # 11 -  Chapter 5b - DNA replication

3.  (10 points)  Explain the role of Escherichia coli RNA Polymerase III and topoisomerase I in replication of the bacterial chromosome.
 
 
 
 
 

Lecture # 12 - Chapter 5c - Methods of DNA Characterisation

4. (10 points) List FIVE different methods for DNA sequencing, and rate them in terms of their speed and efficiency.
 
 
 
 
 
 
 

Lecture # 13 - Chapter 6a - Bacterial Chromosomes
5.  (10 points)  List the major chromatin-associated proteins in Escherichia coli, and explain the role they play in restraining supercoils.
 
 
 
 
 
 
 
 

Lecture # 14 - Chapter 6b - Chromosome Anatomy

6. In class we talked about the tips & middle parts of chromosomes.

6a. (2 pts.) Name the end or "tips" of chromosomes __________________

6b. (3 pts.) What unique type of DNA structure does this bit of the chromosome form?

6c. (3 pts.) What does this have to do with cancer??

6d. (2 pts.) Name the middle-bit of chromosomes __________________
 
 
 
 
 
 
 
 

Lecture # 15 - Chapter 6c - DNA compaction

7. (10 pts.)  List the 5 major histone proteins found in Eukaryotes; include their evolutionary conservation and how the nucleosome octamer is assembled.
 
 
 
 
 
 
 
 

Lecture # 16 - Chapter 6d - Curved DNA

8.  (10 pts.) Within the sequence below, find an area of strongly curved DNA, a region that is flexible.  (There might be more than one correct answer!)

        1 gaattcgatg taattttcag tgtagaccat aattttctta actgaggttc tacgcggctt
       61 tatggacttt tctgaaacgt tgtgaatgcg caatcaggta aggtaggccc gcggttgata
      121 ataacgagga tcaaatatgt atttacgacc agacgaggtg gcgcgcgtac ttgaaaaagt
      181 cggttttact gtcgatgtgg taacccagaa agcgtatggt taccgccgtg gggaaaatta
      241 tgtctatgtt aatcgcgaag cgcgaatggg acgtaccgct ctggtaattc atccaacatt
      301 aaaagaacgc agttcgagac tggctgaacc cgcttccgat attaaaactt gcgatcatta
      361 ccagcaattt ccgctctatt tagcaggcga gcgacacgag cattacggta tcccgctagg
      421 ctttagttgc gtgttgcgct tgaacgttat ttgaatggtt tatttggcga agccagttaa
      481 agacactggc gatggcccga gtacaagaag ggtcaggctt ttacctgctg gtaacggctg
      541 actttaaaca gttttttgca attttcgagg atttttccat atttttcccc catcaacatc
      601 gtttttacga ttttttaact gtttttcgcc attttctgat gtttttctgc gcccaccact
      661 aacaatatat atatatacat atatataata atataccata tataataata tatatcagat
      721 tttttatcca gcccgacggc taactttgcg tgccgcgcgc ataaacagat cacggtacat
      781 accgtatggc catgcaatta agatgttcac cggaatcgct accaatctgg aataaaaaga
      841 ctgttcgaag ctcattccgg agaggaaaac ttcaatacac atgttcacga cagaacagta
      901 aacaaccatc gcgaacgtat ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat
      961 gctaatgctc cttaataaaa gcgtaatggg atgttaatgg agatgaaagc agtggatctc
     1021 attgccatct gcattttcgc atagcgtata tcgctggcta tgactaatca attagtgaaa
     1081 aatttttatt tagtcagttt ttgtagataa aatattccgt aacggctcgt tttttgtaca
     1141 ttgtggattt ttacatgccg atatgattta tttttaatta aatcaattag atatggcctt
     1201 acctttaata tcactaatga aagtttgggg gtgaggtaac gctataagcg tagagaattc
     1261 ggtgtatgta gtagctgggt cattttcagc attttttatg ccagacaatg tacattaaca
     1321 gcagaaagca ccagtgatgg atgttttctt ttattctgtt atattgcatt tttattcact
     1381 ttgattttgt caggcttgcg gaattagcga gcagagagcg ccgctctgtt caccaataat
     1441 ctaaattact catcaaaatg gctaatatat aatcttgaaa ttattctgag agtttcagaa
     1501 aataacagct gaaataatct cgcgcaggac tgtaaataga ttaaattttg tggaaatata
     1561 ataagtgatc gcttacacta cgcgacgaaa tacttttttt gttttggcgt taaaaggttt
     1621 tctttattat gtccgtaatg ttacaaagtt taaataacat tcgcaccctc cgtgcgatgg
     1681 ctcgcgaatt ctccattgac gttcttgaag aaatgctcga aaaattcagg gttgtcacta
     1741 aagaaagacg tgaagaagaa gaacagcagc agcgtgaact ggcagagcgc caggaaaaaa
     1801 ttagcacctg gctggagctg atgaaagctg acggaattaa cccggaagag ttattgggta
     1861 atagctctgc tgctgcacca cgcgctggta aaaaacgcca gccgcgtccg gcgaaatata
     1921 aattcaccga tgttaacggt gaaactaaaa cctggaccgg tcagggccgt acaccgaagc
     1981 caattgctca ggcgctggca gaaggtaaat ctctcgacga tttcctgatc taattactga
     2041 ggccggatgt cgctgctagg gcgcgtccaa catcgcttct caagctcgtt ggttccacgg
     2101 catcttatct aacaaccttg ccattccaca aaaaccgctg attcctgcaa acagtaaccc
     2161 ggcaccaaca aagccactta ataagaagaa accgctattt acggtataac ccagtacaa
 

Lecture # 17 - Chapter 7a - Changes in Chromosome NUMBERs

9.  (10 pts.)  Why are bannannas sterile;  watermelons can be seedless, and how you can avoid "bad oyster" season?
 
 
 
 
 
 

Lecture # 18 - Chapter 7b - Changes in Chromosome STRUCTUREs

10.  (10 pts.) Describe the mechanism (in terms of changes in chromosomal structure) for the mutation commonly associated with colour-blindness.
 
 
 
 
 
 
 

Extra credit:

Explain how you think telomerase gene therapy might work for treating cancer.



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Last modified on: 2 February, 2000 by Dave Ussery